A method for supercritical extraction of cerebrosides from sea cucumbers

A cerebroside and supercritical technology, which is applied in chemical instruments and methods, organic chemistry, and bulk chemical production, can solve the problems of large loss of cerebroside, long purification cycle, and limited research, so as to improve the extraction rate and Purity, efficient and rapid extraction, and high product extraction rate

Active Publication Date: 2019-12-20
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Too many chromatographic steps lead to large loss of cerebroside, low yield, and long purification cycle, which limits its further research

Method used

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  • A method for supercritical extraction of cerebrosides from sea cucumbers
  • A method for supercritical extraction of cerebrosides from sea cucumbers
  • A method for supercritical extraction of cerebrosides from sea cucumbers

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Establishment of detection method for cerebrosides in sea cucumbers

[0038] The cerebroside of the present invention is detected and analyzed by normal phase high performance liquid chromatography-evaporative light scattering detection method. The chromatographic conditions of the normal phase high-efficiency chromatography-evaporative light scattering detection method are: the chromatographic column is an XB-CN column (4.6mm×250mm, 5um), the column temperature is 35°C, and the mobile phase A is n-hexane-isopropanol (99 :1, V / V), the mobile phase is dichloromethane-methanol (60:40, V / V), the gradient elution program is shown in Table 1, the injection volume is 20 μ L, the flow rate is 1.0 mL / min, and the evaporative light scattering detector The temperature of the drift tube is 50°C, the nebulizer is air, and the flow rate is 2.4L / min.

[0039] Table 1 Gradient elution program table

[0040]

[0041] Accurately weigh an appropriate amount of standard cer...

Embodiment 2

[0042] Example 2 Extracting Cerebrosides from Leaf Ginseng

[0043] In the present embodiment, the method for supercritically extracting cerebrosides from ginseng ginseng comprises the following steps:

[0044] (1) Boil fresh leaf ginseng in boiling water for 50 seconds until the surface hardness of leaf ginseng becomes larger and is not easily deformed;

[0045] (2) Slice the boiled ginseng with a thickness of 30mm, put it into a vacuum freeze dryer and dry it to constant weight; the conditions for vacuum freeze drying are: the temperature of the heating plate is 60°C, and the pressure of the drying chamber is 30Pa, after drying for 14 hours, the weight of the material reaches a constant value, that is, the drying is completed, and the material is taken out;

[0046] (3) Put the dried ginseng into a pulverizer for pulverization, and select a 100-mesh sieve to sieve;

[0047] (4) Put 6 g of sieved ginseng into the extraction kettle and turn on the supercritical CO 2 The ext...

Embodiment 3

[0051] Embodiment 3 extracts cerebroside from Haitian melon

[0052] The method for supercritical extraction of Haitian melon cerebrosides in the present embodiment comprises the following steps:

[0053] (1) Boil the fresh Haitian melon in boiling water for 40 seconds until the surface of the Haitian melon becomes hard and hard to deform;

[0054] (2) Slice the boiled Haitian melon with a thickness of 20mm, put it in a vacuum freeze dryer and dry it to constant weight; the conditions for vacuum freeze drying are: the temperature of the heating plate is 59°C, and the pressure of the drying chamber is 29Pa , after drying for 15 hours, the weight of the material reaches a constant value, that is, the drying is completed, and the material is taken out;

[0055] (3) Put the dried Haitian melon into a pulverizer for pulverization, and select a 50-mesh sieve to sieve;

[0056] (4) Put 6g of sieved Haitian melon into the extraction kettle and turn on the supercritical CO 2 The ext...

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Abstract

The invention discloses a method for supercritical extraction of cerebroside in sea cucumber. The method comprises the following steps of boiling the sea cucumber in boiled water, slicing, freeze-drying under the vacuum atmosphere, and crushing; feeding into an extraction kettle, starting supercritical CO2 (carbon dioxide) extraction equipment, pressurizing and heating CO2, and dynamically extracting the cerebroside in the sea cucumber in the extraction kettle in a supercritical liquid state; detecting and analyzing the collected cerebroside extract by a normal phase high performance liquid chromatography-evaporative light scattering detection method. Compared with the traditional extracting method, the method has the characteristics that the cerebroside in the sea cucumber is extracted by the supercritical CO2; the efficiency is high, the operation is quick, the extracting rate of a product is high, the purity of the product is high, any chemical reagent is not used, and the pollution is avoided; by optimizing each condition in the supercritical CO2 extraction, the optimum technology condition of the supercritical CO2 extraction is finally determined; under the optimum technology condition, the extracting rate of the cerebroside reaches 2.54%, and the purity of the obtained cerebroside reaches 11.2%.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for supercritically extracting cerebrosides from sea cucumbers. Background technique [0002] Sea cucumber is a precious marine tonic, rich in various bioactive ingredients, and has high nutritional and medicinal value. Sea cucumber cerebrosides are a class of glycosphingolipids (sphingolipids) with unique structure and activity existing in the body wall of sea cucumbers. Sphingosine base (referred to as long-chain base, LCB) consists of three parts. Although the content of cerebroside in sea cucumber is very small, its biological activity is different from that of other organisms because of its novel structure. Cerebroside compounds mainly have biological activities such as anti-tumor, immune regulation, induction of cell apoptosis, antibacterial, and anti-hepatotoxicity (Jiang Jian, Yang Baoling, Tai Yang. Research on Sea Cucumber Resources and Bioactive Subs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/10C07H1/08
CPCC07H1/08C07H15/10Y02P20/54
Inventor 孙剑锋徐志利代鑫鹏孙兆平牟建楼刘亚琼马艳莉
Owner HEBEI AGRICULTURAL UNIV.
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