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GnRH antigen and application thereof in active immunization affecting castration effect and meat quality of oxen

A technology of antigens and products, applied in the direction of vertebrate antigen components, medical preparations containing active ingredients, antibody medical components, etc., can solve the problems of differences in immune results and unsatisfactory effects, so as to enhance immunogenicity and improve the immune system Recognition ability, enhance the effect of antigen immunogenicity

Active Publication Date: 2017-07-28
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some foreign scholars have carried out experimental studies on GnRH immunized animals, but due to the different structures and monomer numbers of the GnRH haptens they used, there are great differences in the immunization results.
When the GnRH monomer is combined with the carrier protein as an antigen, the effect is not satisfactory after immunizing animals (Sabeur K et al, 2003)

Method used

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  • GnRH antigen and application thereof in active immunization affecting castration effect and meat quality of oxen
  • GnRH antigen and application thereof in active immunization affecting castration effect and meat quality of oxen
  • GnRH antigen and application thereof in active immunization affecting castration effect and meat quality of oxen

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] The preparation of embodiment 1, GnRH vaccine

[0091] 1. Obtaining GnRH derivatives

[0092] GnRH short peptides in nature are composed of 9 kinds of amino acids, namely glutamic acid, histidine, tryptophan, serine, tyrosine, glycine, leucine, arginine and proline. The 6th glycine in the GnRH short peptide was replaced by D-lysine, and four types of GnRH epitopes were designed, including eight GnRH derivatives shown in Table 1. The eight GnRH derivatives were: Molecular GnRH antigen tandem, double cysteine ​​tandem, insertion of a short peptide MAL that is easy to form an α-helix, and insertion of a short peptide VIY that is easy to form a β-sheet (see Table 1 for the sequence of the antigenic epitope).

[0093] Table 1 is the amino acid sequence of GnRH derivatives

[0094]

[0095]

[0096] The eight GnRH derivatives A-H shown in the above Table 1 were synthesized by Shanghai Gil Biochemical Co., Ltd., and purified by high performance liquid chromatography (H...

Embodiment 2

[0107] Embodiment 2, the application of GnRH vaccine

[0108] 1. GnRH vaccine immunization mice small test

[0109] 1. Experimental animals

[0110] Twenty-seven healthy male mice (purchased from the Animal Experiment Center of Shihezi University) of 1 month old and with similar body weight were randomly divided into 9 groups, which were designated as the control group, group A, group B, group C, group D, and group E respectively. Group, Group F, Group G, Group H;

[0111] 2. Immunity

[0112] Immune groups A-H: at the age of 1 month, respectively take the method of leg muscle injection to the 8 groups of male mice of A-H, and correspondingly immunize 1 ml of the 8 kinds of GnRH vaccines A-H (containing about 50 μg of GnRH) obtained in Example 1; The experimental animals were boosted once with the injection method.

[0113] Control group: the experimental animals were immunized with the vaccine emulsified by physiological saline and adjuvant, and the feeding and management...

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Abstract

The invention discloses a GnRH antigen and application thereof in active immunization affecting the castration effect and meat quality of oxen. The invention provides a GnRH derivative which is obtained by inserting oligopeptides, which can form alpha helixes, among multiple serially-connected single GnRH antigens, and each single GnRH antigen is a polypeptide obtained by replacing the sixth-site glycine in the amino acid sequence of gonadotropin releasing hormone GnRH with D type lysine. When a GnRH two-string alpha-helix vaccine screened by experiments is used to actively immunize male rats and the oxen, the vaccine can allow the biological activity of testosterone to be partially or completely lost, an immunocastration effect is achieved, a good immunization enhancing effect is achieved, and the carcass quality of immunized animals can be increased to a certain degree.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a GnRH antigen and its application in the castration effect of active immunization on bulls and the influence on meat quality. Background technique [0002] Castration of livestock is an effective method to control the libido and reproductive capacity of livestock. It not only makes livestock tame and easy to feed and manage, improves feed remuneration, but also improves meat quality and carcass grade. The carcasses of uncastrated boars contain androstenone produced by Leydig cells of the testis, which makes the pork produce a "fishy smell" during the heating process, which seriously affects the quality of pork (Matthews KR et al, 2000); bulls and rams often suffer from temperament The phenomenon of fighting with each other, injuring the same kind and the breeders due to violence, and at the same time, there are still problems such as poor tenderness, rough texture and smell of meat ...

Claims

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Application Information

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IPC IPC(8): C07K7/23A61K39/00A61P15/00
CPCA61K39/0005C07K7/23
Inventor 徐梦思徐春生沈思军陈宁陈红莉盛铭孟季蒙沈敏高磊杨井泉尹君亮甘尚权
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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