Preparation method of blue latex microsphere labeled antibody and test paper strip thereof

A technology of latex microspheres and labeled antibodies, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of weak signal strength, large batch-to-batch variation, single color, etc., and achieve narrow particle size distribution, less antibody dosage, Good dispersion effect

Inactive Publication Date: 2017-07-28
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0005] In view of this, the present invention aims to propose a preparation method of blue latex microsphere-labeled antibody and an immunochromatographic analysis method, which can visualize and quickly detect norfloxacin residues, so as to solve the problem of relatively low cost of the colloidal gold-labeled immunochromatographic analysis method. High, large differences between batches, weak signal strength, single color, etc.

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  • Preparation method of blue latex microsphere labeled antibody and test paper strip thereof
  • Preparation method of blue latex microsphere labeled antibody and test paper strip thereof
  • Preparation method of blue latex microsphere labeled antibody and test paper strip thereof

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[0035] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0036] The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0037] A kind of preparation method of latex microsphere labeled antibody

[0038] 1) Wash 50mg with mes buffer. mL -1 The blue latex microspheres, repeated twice, centrifuged at 14000r / min, 4°C for 15min; dilute the precipitate with mes buffer to 1mg. mL -1 , stored at 4°C for later use;

[0039] 2) Take 200μL of 1mg. mL -1 blue latex microspheres, add 0.5mg﹒ mL -1 The EDC and NHS were reacted on a shaker for 15 minutes;

[0040]3) Wash the activated latex microspheres with phosphate buffer, repeat twice, centrifuge at 14000r / min, 4°C for 15min, and dilute the precipitate to 1mg with phosphate buffer. mL -1 , stored at 4°C for later use;

[0041] 4) Mix 15 μL of 1mg. mL -1...

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Abstract

The invention provides a preparation method of blue latex microsphere labeled antibody and the test strip thereof. The present invention selects norfloxacin as the target object, and adds norfloxacin antibody to the blue latex microsphere solution to obtain Microsphere-labeled antibody, established a blue latex microsphere-labeled immunochromatographic analysis method for the detection of norfloxacin, and applied it to the visual detection of norfloxacin residues in animal-derived foods. The preparation method of the blue latex microsphere-labeled antibody of the present invention and the test strip thereof have the following advantages: (1) in the preparation of the blue latex microsphere-labeled antibody of the present invention, the labeling material used is blue The cost of latex microspheres is much lower than that of chloroauric acid, which reduces the cost of existing pesticide detection. (2) The test strip of the present invention uses less antibody, which also saves the cost. (3) The latex microspheres used in the present invention have the characteristics of good dispersibility, narrow particle size distribution, and bright colors.

Description

technical field [0001] The invention belongs to the field of rapid detection of veterinary drug residues, and in particular relates to a preparation method of blue latex microsphere-labeled antibody and a prepared test strip. Background technique [0002] The immunochromatographic analysis method has the advantages of high sensitivity, simple operation, time-saving and labor-saving, low cost, and is suitable for rapid on-site screening of a large number of samples. It has developed rapidly in the field of veterinary drug residue detection in recent years. Colloidal gold has the advantages of good biocompatibility, different particle sizes can show different colors, and it is easy to improve the signal intensity through modification. It has become the most commonly used labeling material in immunochromatographic analysis methods. However, colloidal gold is expensive and has large batch-to-batch differences, which is not conducive to the mass production of test strips. Colloi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/558
CPCG01N33/54313G01N33/558
Inventor 刘冰孙晓生威张燕王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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