Preparation method of blue latex microsphere labeled antibody and test paper strip thereof
A technology of latex microspheres and labeled antibodies, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of weak signal strength, large batch-to-batch variation, single color, etc., and achieve narrow particle size distribution, less antibody dosage, Good dispersion effect
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[0035] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.
[0036] The present invention will be described in detail below with reference to the accompanying drawings and examples.
[0037] A kind of preparation method of latex microsphere labeled antibody
[0038] 1) Wash 50mg with mes buffer. mL -1 The blue latex microspheres, repeated twice, centrifuged at 14000r / min, 4°C for 15min; dilute the precipitate with mes buffer to 1mg. mL -1 , stored at 4°C for later use;
[0039] 2) Take 200μL of 1mg. mL -1 blue latex microspheres, add 0.5mg﹒ mL -1 The EDC and NHS were reacted on a shaker for 15 minutes;
[0040]3) Wash the activated latex microspheres with phosphate buffer, repeat twice, centrifuge at 14000r / min, 4°C for 15min, and dilute the precipitate to 1mg with phosphate buffer. mL -1 , stored at 4°C for later use;
[0041] 4) Mix 15 μL of 1mg. mL -1...
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