Multicolor stimulated radiation depletion super-resolution imaging device, method and optical microscope

Abstract

A multi-color stimulated radiation exhaustion super-resolution imaging device comprises a plurality of excitation light sources, a loss light source, a photoelectric detector, a control unit and an optical system. The optical system comprises a plurality of shutters which are located on exciting light optical path output by each excitation light source respectively. The control unit makes that the plurality of shutters open by turns so that excitation light output by each excitation light source successively irradiates an imaging area. On the device, multiple paths of excitation light carries out sample excitation and a same path of STED loss light is used to carry out fluorescence quenching so that STED simultaneous multicolor super-resolution imaging is realized and a condition that image deviation is generated among channels is avoided. Besides, in the imaging device, the shutters are used to open and close excitation and detection of the different channels respectively, line scanning mode imaging is realized and a cross interference problem among the plurality of channels is solved.

Description

technical field [0001] The invention relates to the field of microscopic imaging, further relates to a multicolor super-resolution imaging device and a multicolor stimulated radiation depletion super-resolution imaging method, and further relates to an optical microscope based on scanning imaging. Background technique [0002] Since the optical microscope can perform real-time dynamic imaging observation of biological samples under physiological conditions, it has become an indispensable research tool for biologists, and the development of optical microscopes is also accompanied by the progress of life sciences. However, due to the existence of the optical diffraction limit, the spatial resolution of optical microscopy is limited to about half a wavelength, which seriously hinders biologists from conducting finer research at the subcellular scale. Since Stefan W. Hell theoretically proposed to break through the diffraction limit in 1994, after more than 20 years of developme...

AI Technical Summary

Problems solved by technology

However, no matter which method is used to achieve multi-color STED imaging, it will face the problem of cross-interference (cross-color) between multiple channels, and the first method will also have the problem of lateral deviation between multiple imaging channels.

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