Double-drug-loading self-healing hydrogel system and preparation method thereof
A dual drug-loading and hydrogel technology, which is applied in the direction of drug combinations, pharmaceutical formulations, and medical preparations of non-active ingredients, can solve the problems of clinical use restrictions, low oral activity, strong toxic and side effects, etc., and achieve short preparation cycles , good biocompatibility, and the effect of reducing toxic and side effects
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Embodiment 1
[0029] 1) Dissolve 10.000 g pullulan in 400 mL double-distilled water, and stir it with magnetic force to fully dissolve it;
[0030] 2) Add 10 mL of 232 mg / mL oxidant potassium periodate solution to the solution obtained in step 1) and react for 1.5 h;
[0031] 3) Add 0.72 mL glycerol to the solution obtained in step 2) and react for 15 min to terminate the oxidation reaction;
[0032] 4) Place the solution obtained in step 3) in a dialysis bag of MWCO 6000, and dialyze at 4°C for 3 days, during which time the water is changed every 6-8 hours;
[0033] 5) Vacuum freeze-dry the dialyzed solution for 3 days to obtain pure solid oxidized pullulan;
[0034] 6) Accurately weigh 1.000 g of the obtained oxidized pullulan, add 20 mL of double distilled water, and dissolve at 37°C;
[0035] 7) Add 30 mg cisplatin and 40 mg doxorubicin hydrochloride to the solution obtained in step 6), and fully dissolve at 37°C;
[0036] 8) Add 250 μL of 0.5 g / mL ε-polylysine aqueous solution to th...
Embodiment 2
[0041] 1) Dissolve 10.000 g pullulan in 400 mL double-distilled water, and stir it with magnetic force to fully dissolve it;
[0042] 2) Add 10 mL of 232 mg / mL oxidant potassium periodate solution to the solution obtained in step 1) and react for 1.5 h;
[0043] 3) Add 0.72 mL glycerol to the solution obtained in step 2) and react for 15 min to terminate the oxidation reaction;
[0044] 4) Place the solution obtained in step 3) in a dialysis bag of MWCO 6000, and dialyze at 4°C for 3 days, during which time the water is changed every 6-8 hours;
[0045] 5) Vacuum freeze-dry the dialyzed solution for 3 days to obtain pure solid oxidized pullulan;
[0046] 6) Accurately weigh 1.000 g of the obtained oxidized pullulan, add 20 mL of double distilled water, and dissolve at 37°C;
[0047] 7) Add 30 mg cisplatin and 40 mg doxorubicin hydrochloride to the solution obtained in step 6), and fully dissolve at 37°C;
[0048] 8) Add 350 μL of 0.5 g / mL ε-polylysine aqueous solution to the ...
Embodiment 3
[0053] 1) Dissolve 10.000 g pullulan in 400 mL double-distilled water, and stir it with magnetic force to fully dissolve it;
[0054] 2) Add 10 mL of 232 mg / mL oxidant potassium periodate solution to the solution obtained in step 1) and react for 1.5 h;
[0055] 3) Add 0.72 mL glycerol to the solution obtained in step 2) and react for 15 min to terminate the oxidation reaction;
[0056] 4) Place the solution obtained in step 3) in a dialysis bag of MWCO 6000, and dialyze at 4°C for 3 days, during which time the water is changed every 6-8 hours;
[0057] 5) Vacuum freeze-dry the dialyzed solution for 3 days to obtain pure solid oxidized pullulan;
[0058] 6) Accurately weigh 1.000 g of the obtained oxidized pullulan, add 20 mL of double distilled water, and dissolve at 37°C;
[0059] 7) Add 30 mg cisplatin and 40 mg doxorubicin hydrochloride to the solution obtained in step 6), and fully dissolve at 37°C;
[0060] 8) Add 50 μL of 0.5 g / mL ε-polylysine aqueous solution to the...
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