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Nanogel inclined plane culture medium and preparation method thereof

A slant medium and nano-gel technology, which is applied in the field of nano-gel slant medium and its preparation, can solve the problems of affecting the nutritional balance of bacterial growth, the large difference in thickness between the upper and lower sides of the slant, and affecting the accuracy of the test. The effect of avoiding repeated tests, delaying the setting time, and shortening the test time

Active Publication Date: 2017-08-11
句容亿格纳米材料厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the solid medium generally uses agar as the main solidified carrier and supplemented with other nutrients, but there are certain problems when it is applied to the slant medium, because the solidification speed of the agar is too fast at 40°C-45°C , when the solidification time is too short, it is easy to cause uneven solidification and too large thickness difference between the upper and lower sides of the slope when preparing the slant medium. Uneven coating, when the line is too thin, there will be scratches and scratches, which will affect the accuracy of the later test

Method used

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  • Nanogel inclined plane culture medium and preparation method thereof

Examples

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Effect test

Embodiment 1

[0019] A nanogel slant culture medium, used for the cultivation of Staphylococcus aureus, specifically includes the following components: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar powder 7g, nanogel particles 6g, agar Sugar gel 7g; add distilled water to make up to 1L;

[0020] The nanogel particle is the preparation of zinc oxide-titanium dioxide nanocomposite material: dissolve zinc chloride in an acidic solution of dehydrated alcohol ten times the volume, react in a low-temperature plasma reactor for 10 minutes, add titanium dioxide powder and continue the reaction for 20 minutes, The reaction temperature is -12°C. After the reaction is completed, take it out and stir it sufficiently until the liquid phase is evenly dispersed; adjust the pH to 2, and then place it in a low-temperature plasma reactor at -5°C for more than 12 hours to form a uniform sol, then take it out and evaporate it It is dried and transformed into a gel to obtain nanogel part...

Embodiment 2

[0027] A nanogel slant medium, used for the cultivation of Staphylococcus aureus, specifically includes the following components: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 11g, nanogel particles 4g, agarose Gel 5g; add distilled water to make up to 1L;

[0028] The nano gel particle is the preparation of zinc oxide-titanium dioxide nanocomposite material: dissolve zinc chloride in an acidic solution of dehydrated alcohol ten times the volume, react in a low-temperature plasma reactor for 20 minutes, add titanium dioxide powder to continue the reaction for 30 minutes, The reaction temperature is -6°C. After the reaction is completed, take it out and stir it sufficiently until the liquid phase is uniformly dispersed; adjust the pH to 2-3, then put it in a low-temperature plasma reactor and let it stand for more than 12 hours at 0°C to form a uniform sol, then take it out Evaporated and dried into a gel to obtain nanogel particles with a size of less t...

Embodiment 3

[0031] A kind of nano-gel slant culture medium, used for the cultivation of Staphylococcus aureus, specifically includes the following components: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar powder 9g, nano-gel particles 5g, agar Sugar gel 6g; add distilled water to make up to 1L;

[0032] The nano gel particle is the preparation of zinc oxide-titanium dioxide nanocomposite material: dissolve zinc chloride in an acidic solution of dehydrated alcohol ten times the volume, react in a low-temperature plasma reactor for 15 minutes, add titanium dioxide powder to continue the reaction for 25 minutes, The reaction temperature is -9°C. After the reaction is completed, take it out and stir it sufficiently until the liquid phase is uniformly dispersed; adjust the pH to 2.5, then place it in a low-temperature plasma reactor for more than 12 hours at -3°C to form a uniform sol, then take it out and evaporate it It is dried and transformed into a gel to obtain na...

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Abstract

The invention discloses a nanogel inclined plane culture medium and a preparation method thereof and belongs to the field of microculture. Part of agar powder is replaced with part of nanogel particles and agarose gel on the basis of an ordinary inclined plane culture medium. Concretely, by mass percentage, first nanogel particles are used to replace 20-30% of agar powder, and then the agarose gel is used to replace 25%-35% of agar powder. The setting time and setting rate of the culture medium are effectively delayed, the thickness uniformity degree during inclined plane culture medium preparation is improved, the uniform distribution degree of culture medium nutrient substances is also improved, and thus accuracy of culture and subsequent test results is effectively ensured. Repeated testing is avoided, testing time is shortened, errors are reduced, and the precision degree of tests is improved. In addition, the preparation method is reasonable in design and simple and convenient to operate.

Description

technical field [0001] The invention relates to the field of microorganism culture, in particular to a nanogel slant medium and a preparation method thereof. Background technique [0002] Culture medium is the nutrient substrate necessary for the growth, reproduction or accumulation of metabolites of microorganisms or animal or plant cells in the process of microbial fermentation or animal and plant cell culture. The components of a general medium include: carbon sources, nitrogen sources, minerals, and other essential substances, such as substances that cannot be synthesized by certain organisms themselves, such as certain amino acids, vitamins or nucleotides. In addition, pH buffers are often added to the medium to maintain a stable pH. [0003] In the prior art, the solid medium is generally based on agar as the main solidified carrier and supplemented with other nutrients, but there are certain problems when it is applied to the slant medium, because the solidification ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 潘峥嵘
Owner 句容亿格纳米材料厂
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