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A tumor marker related to colorectal cancer and its application

A tumor marker and colorectal cancer technology, applied in the field of tumor markers and applications, can solve problems such as unclear functions of lncRNA, and achieve important promotion and application prospects and far-reaching clinical significance

Active Publication Date: 2020-05-12
CHENZHOU NO 1 PEOPLES HOSPITAL
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

lncRNA can lead to abnormal expression of related genes through epigenetic effects and play an important regulatory role in the invasion and metastasis of colorectal cancer, but the functions of most lncRNAs are still unclear
At present, there is no relevant research report on lncRNA-KAT7

Method used

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  • A tumor marker related to colorectal cancer and its application
  • A tumor marker related to colorectal cancer and its application
  • A tumor marker related to colorectal cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] The kit for auxiliary diagnosis, curative effect prediction and prognosis judgment of colorectal cancer includes:

[0018] (1) Reagents for extracting total RNA from colorectal cancer and cancer disc tissues, including Trizol reagent, chloroform, isopropanol, and DEPC water.

[0019] (2) Reagents used for reverse transcription of total RNA into cDNA: promega reverse transcription kit (GoScript TM Reverse Transcriptase, A5003), including: GoScript TM 5X Reaction Buffer, MgCl 2 , PCR NucleotideMix, Recombinant Ribonuclease Inhibitor, GoScript TM Reverse Transcriptase, Nuclease-Free Water.

[0020] (3) Reagents used for cDNA real-time fluorescent quantitative PCR, including lncRNA-KAT7 real-time fluorescent quantitative PCR specific primers, GAPDH internal reference specific primers, real-time fluorescent quantitative SYBR dye (Roche 4887352001 LightCycler480 SYBR Green I Master), DEPC water.

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Abstract

The invention discloses a tumor marker related to colorectal cancer and application. The tumor marker is lncRNA (long noncoding RNA) and is located on the seventeenth chromosome of chr17: 47785696-47797422, a nucleotide sequence of the lncRNA is shown in SEQ ID NO.1 is named as lncRNA-KAT7. According to the lncRNA-KAT7 sequence, specific real-time fluorescent quantitative PCR primer can be designed and synthesized to be used for preparing preparation for colorectal cancer diagnosis or treating effect prediction. By means of the real-time fluorescent quantitative PCR preparation, expression level of lncRNA-KAT7 is detected in colorectal cancer clinical case specimen, expression of the lncRNA-KAT7 in the colorectal cancer is found to be obviously reduced, the lncRNA-KAT7 has obvious statistic significance (p<0.05), and low expression of the lncRNA-KAT7 in the colorectal cancer is closely related to colorectal tumor differentiation, T staging, invasion and metastasis. The result shows that the lncRNA-KAT7 can serve as the tumor marker of the colorectal cancer and can be used for preparing preparation or kits applied to auxiliary diagnosis, treating effect prediction and prognosis of the colorectal cancer.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and relates to a tumor marker related to colorectal cancer and its application. Background technique [0002] Colorectal cancer (CRC) is a highly lethal cancer with the third highest incidence of malignant tumors in the world, and it is still a hot spot of health concern worldwide. Despite continuous advances in the diagnosis and treatment of colorectal cancer, more than 1.2 million new CRC cancer cases and more than 600,000 deaths are recorded each year. The occurrence of CRC is not only closely related to heredity, age, and environmental factors, but also related to factors such as bad eating habits, obesity, and smoking. The specific pathogenesis is still not very clear. Metastatic disease is the leading cause of death in colorectal cancer patients. According to official statistics, the average annual survival rate of patients with focal CRC is as high as 90%, but only 6% for patients ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 罗迪贤贺荣章王晴美胡政罗伟濠李佳段丽丽罗文娜
Owner CHENZHOU NO 1 PEOPLES HOSPITAL
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