A ratiometric fluorescent sensor for measuring cysteine, its preparation method and application
A ratiometric fluorescence, cysteine technology, applied in the field of organic-inorganic composite cysteine ratio fluorescence sensor, can solve the problems of fluorescence signal data distortion, measurement result error, etc., achieve long storage time, low detection limit, Anti-aging effect
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[0035] 1. Preparation of hydrotalcite precursor: 0.008mol Mg(NO 3 ) 2 ·6H 2 O, 0.04mol Al(NO 3 ) 3 ·9H 2 O and 0.048mol urea were dissolved in 280ml of deionized water, placed in four 90ml pressure reaction vessels with a polytetrafluoroethylene substrate, reacted at 110°C for 24 hours, and washed with deionized water by centrifugation to a pH of about It is dried in air at 7,60°C to obtain carbonate-type anion intercalation hydrotalcite crystallites. Take 0.3g of the above carbonate-type anionic hydrotalcite and solid NaNO 3 63.75g dissolved in 300mL in addition to CO 2 After being uniformly dispersed in deionized water, adding 0.08 mL of concentrated nitric acid to acidify and stirring at 25° C. under nitrogen atmosphere to perform ion exchange reaction. In addition to CO after 48 hours 2 The deionized hot water was centrifuged and washed to pH 7, and dried at 60°C for 12 hours to obtain hydrotalcite intercalated with magnesium aluminum nitrate anion.
[0036] 2. Take 0.3 g of...
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