A kind of hepatitis B virus surface antigen gene and expression vector and cell line
A surface antigen gene, hepatitis B virus technology, applied in the direction of virus antigen components, virus/phage, virus, etc., can solve the problems of unstable insertion sequence, affecting antigen expression, and risk of productive expression.
Active Publication Date: 2021-01-29
广州创瑞健康科技有限公司
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Problems solved by technology
[0003] Some inventions (such as the patent application with publication number CN 102851313 A) describe the use of expression vectors (containing nucleoplasmic attachment region sequences) that remove most of the hepatitis B virus core antigen genes, because there are strong upstream promoters such as SV40 in the vectors , will interfere with the ratio of LargeS:S of hepatitis B virus, thereby affecting the expression of the antigen; and because it is randomly inserted into the genome, it will cause instability of the inserted sequence, which is risky for productive expression
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Embodiment 1
[0031] 1. Component selection:
[0032] (1) The sequence of the big S (preS1+preS2+S) antigen gene is selected from the HBV sequence obtained from Guangdong medical clinical samples:
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The invention discloses a hepatitis B virus surface antigen gene, expression vector and cell line. The nucleotide sequence of the hepatitis B virus surface antigen gene is shown in SEQ ID NO.1. The gene only contains part of the core antigen gene sequence and part of the X gene. As a gene for producing vaccines, the safety is better. After the hepatitis B virus surface antigen gene is integrated into the mammalian cell genome for expression, the large antigen can be secreted and expressed, and is suitable for producing the hepatitis B virus surface antigen vaccine.
Description
technical field [0001] The invention belongs to the field of vaccine production, in particular to a hepatitis B virus surface antigen gene, an expression vector and a cell line. Background technique [0002] The method of optimizing the large S antigen gene sequence of hepatitis B virus and constructing a high-efficiency expression vector cannot express secreted HBsAg efficiently. This is because excessive expression of the LargeS gene will inhibit the secretion of surface antigens, and only the correct ratio of LargeS:S can obtain high-efficiency expression of surface antigens, and the regulatory sequence of the S antigen gene is located in the preS sequence, so change its gene It is not advisable to obtain high expression of the large S antigen. [0003] Some inventions (such as the patent application with publication number CN 102851313 A) describe the use of expression vectors (containing nucleoplasmic attachment region sequences) that remove most of the hepatitis B vir...
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IPC IPC(8): C12N15/51C12N15/867C12N5/10A61K39/29A61P31/20A61P1/16
CPCA61K39/12C07K14/005C12N5/0609C12N15/86C12N2510/00C12N2730/10122C12N2730/10134C12N2730/10143Y02A50/30
Inventor 赵金红李军涛
Owner 广州创瑞健康科技有限公司