Application method of long-chain dicarboxylic acid synthesized by thermophile bacteria strain
A technology of long-chain dibasic acid and dodecane dibasic acid, which is applied in the field of bioengineering to achieve the effects of mild conditions, environmentally friendly process and simple process
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Embodiment 1
[0028] (1) The collected soil samples were directly spread on the n-dodecane selection medium by the dilution coating method, and cultured in a water bath at 30° C. for 3 to 5 days. According to the morphology of the colony, select a single colony with differences and inoculate it into the corresponding liquid medium, and culture it in a water bath shaker at the same temperature for 3 to 5 days.
[0029] (2) Select the bacterial strain that can grow on the n-dodecane selective medium, that is, the medium with alkanes as the only carbon source, and perform streak culture on the indicator medium containing methyl red indicator.
[0030] (3) At the same time, pick a single colony that can grow on the selection medium, inoculate it in 50mL indicator medium without adding methyl red indicator, and carry out shake flask culture at 30°C, and on the first day of culture From the beginning, take out 1mL of the culture medium every day, and add 1-2 drops of methyl red indicator dropwise...
Embodiment 2
[0032] (1) The collected soil samples were directly coated on the n-dodecane selective medium by the dilution coating method, and cultured in a water bath at 37° C. for 3 to 5 days. According to the morphology of the colony, select a single colony with differences and inoculate it into the corresponding liquid medium, and culture it in a water bath shaker at the same temperature for 3 to 5 days.
[0033] (2) Select the bacterial strain that can grow on the n-dodecane selective medium, that is, the medium with alkanes as the only carbon source, and perform streak culture on the indicator medium containing methyl red indicator.
[0034] (3) At the same time, pick a single colony that can grow on the selection medium, inoculate it in 50mL indicator medium without adding methyl red indicator, and carry out shake flask culture at 37°C respectively, and in the first culture From the first day, take out 1 mL of the culture medium every day, and add 1-2 drops of methyl red indicator d...
Embodiment 3
[0036] (1) The collected soil samples were directly coated on the n-dodecane selective medium by the dilution coating method, and cultured in a water bath at 45° C. for 3 to 5 days. According to the morphology of the colony, select a single colony with differences and inoculate it into the corresponding liquid medium, and culture it in a water bath shaker at the same temperature for 3 to 5 days.
[0037] (2) Select the bacterial strain that can grow on the n-dodecane selective medium, that is, the medium with alkanes as the only carbon source, and perform streak culture on the indicator medium containing methyl red indicator.
[0038] (3) At the same time, pick a single colony that can grow on the selection medium, inoculate it in 50mL indicator medium without adding methyl red indicator, and carry out shake flask culture at 45°C respectively, and in the first culture From the first day, take out 1 mL of the culture medium every day, and add 1-2 drops of methyl red indicator d...
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