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SNP molecular marker for dividing corn dominant groups and application thereof

A molecular marker and heterosis technology, applied in the field of molecular biology, can solve the problems of high cost, large quantity, and no division of maize dominant groups, and achieve the effects of low cost, improved utilization efficiency, and avoidance of testing and matching work.

Active Publication Date: 2017-08-18
SHENYANG AGRI UNIV
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  • Application Information

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Problems solved by technology

[0004] However, the number of SNP markers used in the above research is in the range of 56k-1k, which is large in number and high in cost. At present, there is no research on the market that uses hundreds of SNP molecular markers to divide dominant groups in maize

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  • SNP molecular marker for dividing corn dominant groups and application thereof
  • SNP molecular marker for dividing corn dominant groups and application thereof
  • SNP molecular marker for dividing corn dominant groups and application thereof

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Embodiment Construction

[0020] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0021] 1. Test materials

[0022] In this experiment, 44 maize inbred lines were used as research materials. The sources of materials include 8 control materials of classical heterosis groups and 36 breeding inbred lines in Liaoning Province. See Table 1. It mainly serves the breeding companies in Liaoning Province and provides basic genetic relationship data.

[0023] Table 1 Name and source of test materials

[0024]

[0025]

[0026] 2. SNP genotyping

[0027] The DNA of 44 maize samples was extracted by CTAB method and teste...

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Abstract

The invention belongs to the technical field of molecular biology, and discloses an SNP molecular marker for dividing corn dominant groups and application thereof. The molecular marker comprises 101 SNP markers covering 10 corn chromosomes, the number of the first chromosome, the number of the second chromosome, the number of the third chromosome, the number of the fourth chromosome, the number of the fifth chromosome, the number of the sixth chromosome, the number of the seventh chromosome, the number of the eighth chromosome, the number of the ninth chromosome and the number of the tenth chromosome are 22, 13, 3, 9, 6, 5, 22, 8, 5 and 5 respectively. At present, the number of the SNP markers for hybrid dividing is 1k-56k, the number is large, cost is high, only 101 SNP markers are used in the method for dividing the hybrid dominant groups, and the effect is the same as the dividing effect of 56k corn SNP chips. Detection cost can be reduced by using the distributed method for dividing the hybrid dominant groups.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP molecular marker for dividing dominant groups in maize and an application thereof. Background technique [0002] Maize is a typical outcross crop exhibiting extreme inbreeding depression and heterosis. Utilization of heterosis is an effective way to greatly increase maize yield and improve quality. Rationally dividing the dominant groups of inbred lines is an important basic research for constructing the utilization mode of maize heterosis, effectively guiding the selection of maize inbred lines and improving the efficiency of combining maize hybrids. [0003] With the development of gene chip technology and sequencing technology, SNP markers have become the most promising third-generation molecular markers after RFLP and SSR. At present, the SNP chips widely used in corn have SNP sites of different densities such as 56k and 1k, which can be used to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 阮燕晔崔震海张敖姜思奇张立军
Owner SHENYANG AGRI UNIV