An electrophoretic film pipetting device for anti-eb staining liquid carcinogenic and wounding for molecular research on soybean aphids

Abstract

The invention relates to an electrophoresis film shifting device for preventing carcinogenic wounding of EB dye liquor for a molecular research of soybean aphids and belongs to the technical field of entomology. The electrophoresis film shifting device for preventing carcinogenic wounding of EB dye liquor for the molecular research of soybean aphids is composed of a grabbing cutter (1) and a tray moving box (44). An electrophoresis film is taken up by means of the grabbing cutter and is placed in the tray moving box, is transferred to a dyeing vat, is then taken up from the dyeing vat by means of the grabbing cutter again if being dyed, is placed in the tray moving box, and then is transferred to a photographing observation dark box; an ultraviolet lamp is turned on for photographing, observing and recording; and then, the whole film is cut down in the device by means of the cutter for follow-up experiments such as identification. The whole process is finished in the device, so that a human hand is prevented from being in direct contact with the EB dye liquor, and therefore, purposes of preventing the human hand from being in direct contact with the EB dye liquor to be carcinogenic to wound, safely transferring the film and reducing the experimental expenditure are achieved. The device is simple to manufacture, high in operability, low in cost and obvious in effect.

Description

technical field [0001] The invention relates to an electrophoretic film transfer device for preventing cancer and wounding caused by EB dye solution for molecular research on soybean aphids, and belongs to the technical field of entomology. Background technique [0002] Soybean aphids belong to the family Aphididae of the order Homoptera, which mainly damage soybeans at the seedling stage. They are distributed in the main soybean producing areas of my country and cause huge losses to soybean production (https: / / baike.sogou.com / v8542710.htm? fromTitle=Soybean aphids. Bean soybean aphid, China Agricultural Resources Online, 2011-02-19). Soybean aphids first appear in mid-to-late June, and the peak period of damage is from the end of June to the beginning of July. Generally, aphids are prone to occur in large numbers in dry weather with little rain and continuous high temperature. Soybean aphids are divided into two species, namely, the wingless viviparous female aphid and the ...

AI Technical Summary

Problems solved by technology

But up to now, the protective measures commonly used by experimenters during use are only wearing latex gloves. In laboratories with better experimental conditions, they wear double gloves, and those that are close to it only wear one layer of gloves.

The method of using the EB dye solution is: 10ulEB+200ml water (Sogou asked: DNA crude extraction experiment procedure? 2010-02-05), dye for 15 minutes, then put on gloves and directly take the film out of the dye tank with bare hands, put it in In the camera device, if the camera device is not in the laboratory, many people will walk around the laboratory building with the dyed film and let the dye drip onto the ground. For this reason, many people in the know have repeatedly reminded or stopped this However, there is no relevant equipment in the laboratory to better protect, even if the instructors want to protect, there is nothing they can do, which poses a great threat to the health of the experimenters

Moreover, the gloves are disposable, thrown away after use, and need to be processed separately, which requires a lot of cost and labor. It is often seen that a molecular laboratory has a box of used gloves in a few days that are regarded as harmful. Garbage is disposed of, so gloves are a big expense for some molecular labs

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