Molecular marker related to grain yard seed number character and detection primer and application thereof

A millet and grain count technology, applied in the field of molecular markers and detection primers, can solve the problems that have not been reported

Active Publication Date: 2017-08-25
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on QTL (quantitative trait locus) mapping and SNP markers related to quantitative traits of millet is mainly focused on the development and utilization of SSR markers, and the use of population inbred line genome sequencing to detect single nucleotide polymorphism (SNP) molecular markers The development and application research has not been reported yet

Method used

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  • Molecular marker related to grain yard seed number character and detection primer and application thereof
  • Molecular marker related to grain yard seed number character and detection primer and application thereof
  • Molecular marker related to grain yard seed number character and detection primer and application thereof

Examples

Experimental program
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Embodiment 1

[0029] Example 1: High-throughput sequencing and SNP marker information analysis

[0030] In this example, the materials of the male parent "Zhanggu No. 3" and the female parent "A2", the F1 generation materials obtained by crossing the two, and the 441 materials of the F2 population obtained after 13 generations of self-crossing of the F1 generation, that is, recombinant inbred lines (Recombinant inbred lines, RILs). Each material was planted in Zhangjiakou, Hebei, and the trait data such as the number of grains was recorded and sorted out. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the trait data of the number of coded grains, the associated SNP markers were obtained, and the SNP markers were verified by clonal sequencing.

[0031] In this example, the RADseq method was used to extract the genomic DNA of each sample individual (including 441 RILs, 2 parents, a...

Embodiment 2

[0060] Example 2: SNP marker verification

[0061] According to the predicted bin interval and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select primers that amplify normally and PCR products conform to the predicted size of the amplification product, recover the product and perform sequencing, and select labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials.

[0062] The predicted bin markers contain multiple SNP sites, and these sites are screened according to the PCR results.

[0063] First, according to the sequencing results after PCR product recovery, the markers with SNP site differences in the amplified gene sequences of the paternal and maternal materials were selected. Specific steps...

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Abstract

The invention discloses a molecular marker related to the grain yard seed number character and a detection primer and application thereof. The molecular marker is CGN-06-168 and is located at the position 5236168bp of the sixth chromosome, and the basic group is C/T. By means of the molecular marker and the detection primer thereof, the grain yard seed number can be predicted, and molecular auxiliary technical support is provided for early-stage authenticating, screening and breeding of the grain yard seed number character.

Description

technical field [0001] The invention relates to the technical field of millet breeding, in particular to a molecular marker related to the grain number trait of millet, detection primers and applications thereof. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Corn ears are composed of cobs and many grain yards. The yardage and grain number are important factors affecting the yield of millet. The study of the position and function of genes related to the control of yield traits such as millet yardage and grain number will be of great help in guiding Millet genetics and breeding are of great significance. [0004] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a DNA sequence polym...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海赵芳范光宇魏玮史高雷张耕耘张晓磊邱凤仓苏旭
Owner ZHANGJIAKOU ACAD OF AGRI SCI
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