Bacillus natto with MK-7 production capacity and application of bacillus natto

A technology of Bacillus natto and Bacillus bean, which is applied in the field of bioengineering, can solve the problems of increasing atherosclerosis and blood pressure, and achieve the effect of improving osteoporosis.

Inactive Publication Date: 2017-09-01
山东凤凰生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the domestic prevention of osteoporosis is simply by supplementing calcium and increasing the intake of calcium from the intestinal tract to the blood. sclerosis, high blood pressure, stroke, arthritis, kidney stones and other diseases

Method used

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  • Bacillus natto with MK-7 production capacity and application of bacillus natto
  • Bacillus natto with MK-7 production capacity and application of bacillus natto
  • Bacillus natto with MK-7 production capacity and application of bacillus natto

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Primary screening of Bacillus natto

[0044] 1 Materials and methods:

[0045] 1.1 Experimental strains, Bacillus natto BLCC1-0048, Bacillus natto BLCC1-0053, Bacillus natto BLCC1-0054;

[0046] 1.2 Fermentation medium and culture conditions:

[0047] Two media formulations:

[0048] Seed medium: glucose 0.2%, peptone 1%, beef extract 0.5%, NaCl 0.5%, pH 7.2-7.4;

[0049] Fermentation medium: yeast extract 5%, glycerol 5%, peptone 10%, K 2 HPO 4 0.06%;

[0050] Culture conditions: culture on a shaker at 37°C for 24h, with a rotation speed of 120rpm, and then culture at 45°C for 5 days.

[0051] 1.3 After the fermentation is finished, the fermentation broth and bacteria are separated by centrifugation, and the bacteria are freeze-dried.

[0052] 1.4 Determination of MK-7 content in fermentation cells and fermentation broth:

[0053] Weigh an appropriate amount of freeze-dried bacteria (about 25 mg), extract it with a mixed solution of chloroform: n-hexane = 2:1 ...

Embodiment 2

[0063] Determination of MK-7 content in Bacillus natto fermented for 6 days under different medium conditions:

[0064] 1 Materials and methods:

[0065] 1.1 Experimental strain Bacillus natto strain BLCC 10053;

[0066] 1.2 Fermentation medium and culture conditions:

[0067] Three basal media formulations:

[0068] N1: Glycerol 3%, Peptone 10%, NaCl 0.5%, K 2 HPO 4 0.02%, pH 7.0-7.2N2: glucose 0.2%, yeast extract 0.5%, peptone 1.0%, sodium chloride 0.5%, pH 7.0-7.2N3: yeast extract 2.5%, glycerol 2.5%, peptone 9.5% , K 2 HPO 4 0.03%, pH 7.0-7.2 Culture conditions:

[0069] The specific experimental scheme is shown in Table 2. The activated Bacillus natto was inserted into the seed medium, cultured on a shaking table at 42°C for 16 hours, transferred to the fermentation medium with an inoculum size of 5%, and cultured on a shaking table at 37°C for 1 day. Transfer to a 45°C incubator for static culture for 5 days.

[0070] 1.3 After the fermentation is finished, th...

Embodiment 3

[0079] Content of Bacillus natto MK-7 under different fermentation time conditions:

[0080] 1 Materials and methods:

[0081] 1.1 Experimental strain Bacillus natto strain BLCC 10053;

[0082] 1.2 Fermentation medium and culture conditions;

[0083] Seed medium N2: glucose 0.2%, peptone 1.0%, sodium chloride 0.5%, yeast extract 0.5%, pH 7.0-7.2; fermentation medium N1: peptone 10%, glycerol 3%, NaCl 0.5%, K 2 HPO 4 0.02%, pH7.0-7.2.

[0084] The activated Bacillus natto was inserted into the seed medium, cultured on a shaker at 42°C for 16 hours, transferred to the fermentation medium at an inoculum size of 5%, cultured on a shaker at 37°C for 1 day, and then transferred to an incubator at 45°C for standing Culture 3d, 4d, 5d, 6d, 7d.

[0085] 1.3 After the fermentation is finished, the fermentation product and bacteria are separated by centrifugation, and the bacteria are freeze-dried.

[0086] 1.4 Determination of MK-7 content in fermented bacteria and fermented liqui...

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Abstract

The invention discloses bacillus natto with MK-7 production capacity and application of bacillus natto. The bacillus natto is named as bacillus natto BLCC1-0053 and was already preserved at China Center for Type Culture Collection on January 16, 2014 with a preservation number of CCTCC M 2014028. The bacillus natto BLCC10053 has the capacity for producing MK-7, the MK-7 is one of conventional active ingredients for vitamin K2, is easy to absorb by the human body, can improve the bone health, prevent the osteoporosis, inhibit the angiosteosis, maintain the arterial elasticity, prevent the arteriosclerosis and protect the cardiovascular health. The bacillus natto can produce MK-7, the yield reaches up to 3.68733mg / g, and the VK2 needed by the metabolism of the human body can be supplemented, so that an effect for preventing and treating various diseases can be achieved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacillus natto capable of producing MK-7 and an application thereof. Background technique [0002] Osteoporosis (OP) is characterized by decreased bone mass and microarchitectural degeneration of bone tissue (thinning of cancellous bone trabeculae, reduced number of fractures, porous and thinned cortical bone), resulting in increased bone fragility and A systemic bone disorder in which the risk of fracture is increased. The cause of the disease is generally believed to be related to calcium and vitamin deficiency, endocrine disorders, nutritional disorders, and insufficient exercise and light. It can occur in any population and at any age, and it is more common in middle-aged and elderly people, especially menopausal women. The serious consequence of osteoporosis is fractures. Osteoporotic fractures can hinder the recovery process of patients, increase medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/66C12R1/07
CPCC12N1/20C12P7/66C12N1/205C12R2001/07
Inventor 单宝龙王静任宝涛张颜廷刘虹刘中青
Owner 山东凤凰生物科技股份有限公司
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