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A kind of preparation method and application of Spirogyra ethanol extract

A technology of water extract and Spirogyra, applied in the field of marine organisms, can solve the problems such as the antioxidative and antiaging effects of Spirogyra that have not been seen, and achieve the effects of short cycle, prolongation of average lifespan, and less consumption of organic solvents.

Active Publication Date: 2020-12-25
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching, there is no report about the anti-oxidation and anti-aging effects of Spirogyra

Method used

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  • A kind of preparation method and application of Spirogyra ethanol extract
  • A kind of preparation method and application of Spirogyra ethanol extract
  • A kind of preparation method and application of Spirogyra ethanol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of Spirogyra ethanol extract

[0037] S1. Take fresh Spirogyra wet algae, freeze at -40°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, centrifuge at a speed of 10,000r / min for 10min, and remove the supernatant Separate from the precipitate, and take the precipitate for subsequent use;

[0038] S2. To the precipitation described in step S1, according to the solid-to-liquid ratio of precipitation and ethanol 1g / mL, add ethanol with a volume fraction of 80% for ultrasonic extraction for 3 times, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. The time is 25 minutes, centrifuged for 15 minutes under the condition of rotating speed of 10000r / min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved with first-grade water to adjust its relative to Spirogyra (Wet algae) The weight concentrati...

Embodiment 2

[0039] Example 2 Preparation of Spirogyra ethanol extract

[0040]S1. Take fresh Spirogyra wet algae, freeze at -20°C for 12 hours, thaw at room temperature for 10 hours, repeat freezing and thawing 5 times, centrifuge at a speed of 5000r / min for 15min, and remove the supernatant Separate from the precipitate, and take the precipitate for subsequent use;

[0041] S2. Add ethanol with a volume fraction of 70% to the precipitate described in step S1 according to the solid-to-liquid ratio of the precipitate to ethanol of 0.8 g / mL for ultrasonic extraction 3 times, the frequency of ultrasonic extraction is 35 kHz, and the temperature is 4 ° C. The time is 30min, centrifuged at 8000r / min for 15min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved in first-grade water, and its relative water ratio is adjusted. The weight concentration of Spirogyra (wet algae) is 1gA / mL (A means Spirogyra ...

Embodiment 3

[0042] Example 3 Preparation of Spirogyra ethanol extract

[0043] S1. Take fresh Spirogyra wet algae, freeze at -30°C for 10 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, centrifuge at 8000r / min for 15min, and remove the supernatant Separate from the precipitate, and take the precipitate for subsequent use;

[0044] S2. Add ethanol with a volume fraction of 90% to the precipitation described in step S1 according to the solid-to-liquid ratio of the precipitation to ethanol of 1.5g / mL for ultrasonic extraction twice, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. The time is 20min, centrifuged at 8000r / min for 10min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, and the product after vacuum drying is dissolved in first-grade water, and its relative water ratio is adjusted. The weight concentration of Spirogyra (wet algae) is 1gA / mL (A means Spirogyra wet algae), ...

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Abstract

The invention belongs to the technical field of marine organisms, and provides a preparing method and application of a spirogyra ethanol extract. The preparing method of the spirogyra ethanol extract comprises the following steps of taking fresh and wet spirogyra to conduct repeated freezing and thawing, after centrifugation is conducted, taking precipitate and adding ethanol with the volume fraction being 70%-90% to conduct ultrasonic extraction, and conducting centrifugation and taking supernatant, so that the spirogyra ethanol extract is obtained. The spirogyra ethanol extract is stable in effective ingredient, can remarkably prolong the average life of caenorhabditis elegans at natural conditions and oxidative stress conditions, promote the elimination of reactive oxide species (ROS) in the body, remarkably lower the level of reactive oxide species (ROS) in the body, has a remarkable antioxidation and anti-aging effect, and can be applied to preparing healthcare foods and drugs with the antioxidation and / or anti-aging function. The preparing method of the spirogyra ethanol extract is simple and feasible, small in organic solvent consumption and suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a preparation method and application of ethanol extract of Spirogyra. Background technique [0002] Spirogyra belongs to Chlorophyta, Chloropsida, Order Zygnematales, Family Zygnemaceae. Spirogyra is the general term for green algae of the genus Spirogyra, also known as water moss, stone coat, water coat, water moss, etc. It is a eukaryotic multicellular algae that generally lives in fresh water. The filaments formed are mostly clusters in water. There are more than 400 species of Spirogyra in the world, which are widely distributed in ponds, ditches, rivers, lakes and rice fields. Spirogyra regenerates with broken filaments or conjugative reproduction, and germinates into new individuals from spores, which spread with water. Spirogyra has strong vitality and fecundity. [0003] Spirogyra can be used as bait for some fishes and can be dried as food. At pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/05A61P39/06A23L33/105
CPCA23V2002/00A61K36/05A61K2236/331A61K2236/333A23V2200/30A23V2200/302
Inventor 黄泽波张琪宋立荣胡丽莉屈思雨
Owner GUANGDONG PHARMA UNIV