A preparation method of a thermosetting elastomer tissue engineering scaffold with a multi-level pore structure

Abstract

The invention relates to a preparation method of a thermosetting elastomer tissue engineering scaffold with a multi-level porous structure. The preparation method comprises the following steps of: (1) mixing a thermosetting material with a filling material to obtain a mixed material; establishing a model of a cuboid netty structure by means of CAD (Computer Aided Design) software; and then adding the mixed material into a heating cavity of a 3D printer and performing 3D printing to obtain an initial scaffold; and (2) performing thermal crosslinking or optical crosslinking on the initial scaffold in the step (1) to obtain the thermosetting elastomer tissue engineering scaffold; and finally, removing the filling material to obtain the thermosetting elastomer tissue engineering scaffold with the multi-level porous structure. The invention solves the fundamental problem of directly printing thermosetting material by thermoplastic FDM (Fused Deposition Modeling), and the prepared tissue engineering scaffold has the multi-level porous structure which is controllable in precision in structure. The method is simple and convenient and suitable for various biological materials and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffolds, in particular to a method for preparing a thermosetting elastomer tissue engineering scaffold with a multi-level pore structure. Background technique [0002] Tissue and organ damage is one of the major diseases that seriously threaten human health. Traditionally, it is mainly treated through clinical organ transplantation and other means. Professor Yuan-Cheng Fung, a pioneer of bioengineering in the 1980s, first coined the term tissue engineering, whose purpose is to generate replaceable tissues or organs with three-dimensional structures in vivo or in vitro to repair, regenerate damaged or lost Human tissues and organs, so as to break through the limitations of existing clinical medical methods on the treatment of damaged tissues or organs, including the limited number of donated organs, allogeneic rejection, potential virus infection, autologous "repairing trauma with trauma" second...

AI Technical Summary

Problems solved by technology

FDM printing technology has been used and researched as a thermoplastic material processing method because of its fusion forming principle, and it is difficult to apply to the above-mentioned thermosetting elastomer materials

Taking PGS as an example, the main difficulty is: first, the prepolymer is a thermoplastic material that can withstand plastic processing and has printability, and then needs further high temperature and vacuum environment for crosslinking and curing

However, in the second step, since the prepolymer itself is sensitive to heat, the heat can greatly increase its fluidity, and it will deform before cross-linking and curing to destroy the original structure. In the end, it is impossible to process PGS by 3D printing. cross-linked elastomer

Due to the incompatibility between the above-mentioned properties of thermosetting materials and the principle of FDM thermoplastic processing, there have been no reports of using FDM to print thermosetting bioscaffolds, let alone FDM printing of PGS elastomeric scaffolds.

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