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A kind of immune checkpoint inhibitor pharmaceutical composition targeting CD47 and preparation method thereof

A technology of immune checkpoints and inhibitors, applied in the biological field, can solve problems such as autophagy inhibitors that have not yet been seen

Active Publication Date: 2022-01-04
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports of new preparations composed of autophagy inhibitors and fusion proteins targeting CD47

Method used

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  • A kind of immune checkpoint inhibitor pharmaceutical composition targeting CD47 and preparation method thereof
  • A kind of immune checkpoint inhibitor pharmaceutical composition targeting CD47 and preparation method thereof
  • A kind of immune checkpoint inhibitor pharmaceutical composition targeting CD47 and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0025] 1) Configuration of autophagy inhibitor drugs

[0026] (1) Preparation of chloroquine solution: Dissolve an appropriate amount of chloroquine in 0.01M PBS (pH=7.4) to form a 10mmol / L storage solution, filter and sterilize with a 0.1μm filter and store at 4°C, dilute 500- 1000 times for inhibiting autophagy;

[0027] (2) Preparation of ammonium chloride solution: Dissolve an appropriate amount of ammonium chloride in 0.01M PBS (pH=7.4) to make a 0.4mol / L storage solution, filter and sterilize with a 0.1μm filter, store at 4°C, and in vitro Diluted 50-80 times in the experiment to inhibit cell autophagy;

[0028] (3) Preparation of 3-MA solution: Dissolve an appropriate amount of 3-MA in 0.01M PBS (pH=7.4) to prepare a 200mM stock solution, filter and sterilize with a 0.1μm filter, and store at -20°C. Diluted to 2mM to inhibit cell autophagy; (4) Preparation of LY294002 solution: Dissolve an appropriate amount of LY294002 in 0.01M PBS (pH=7.4) to prepare a 20mM stock so...

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Abstract

The invention belongs to the field of biological technology, and relates to the preparation and application of a CD47-targeted immune checkpoint inhibitor pharmaceutical composition. The present invention consists of an immune checkpoint inhibitor targeting CD47 and a cell autophagy inhibitor to form a combined medicine or a medicine compound preparation. The autophagy inhibitor can enhance the killing effect of drugs targeting CD47 on tumor cells, thereby enhancing the anti-tumor efficacy of such drugs; the compound preparation of the combination of the autophagy inhibitor and the drug targeting CD47, It can enhance the targeting effect of the drug, as well as the blocking effect on the CD47 site and the anti-tumor effect, while ensuring the stability and targeting of the original immune checkpoint inhibitor targeting CD47. The preparation can be used in the diagnosis, detection and treatment of various tumors. The preparation process of the preparation is simple, has wide adaptability, and can be used for large-scale production.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a novel immune checkpoint inhibitor pharmaceutical composition targeting CD47 and its preparation method and application, especially an immune checkpoint inhibitor targeting CD47 and one or more cells Novel compound medicine or pharmaceutical composition formed by combination of autophagy inhibitors and its preparation method and application. Background technique [0002] The prior art discloses that CD47 (also known as integrin-associated protein, integrin associated protein IAP) is a cell transmembrane protein highly expressed on the surface of tumor cells, which includes five transmembrane domains and an Ig-like domain; In the immune cells of the body, the ligand of CD47 is mainly Signal Regulatory Protein α (SIRPα, also known as MyD-1); CD47 regulates cell migration, phagocytosis and immune homeostasis by binding to its ligand; in the immune system of the body , CD47 plays...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K38/17A61K31/4706A61P35/00
CPCA61K38/1774A61K45/06A61K31/4706A61K2300/00
Inventor 鞠佃文章旭耀范佳君李玉彬王绍飞王子玉宋平栾静韵王一辰陈其成
Owner FUDAN UNIV