Method for preparing double-layer membrane for spinal cord treatment

A production method and double-layer film technology, applied in the direction of non-active ingredient medical preparations, active ingredient-containing medical preparations, pharmaceutical formulations, etc., can solve the problems of directional drug release and low drug loading, and achieve the improvement effect Effect of Potency, Warranty Time and Concentration

Inactive Publication Date: 2017-09-29
无锡市锡山人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] 2. Provide a new manufacturing method for the drug-loading system to

Method used

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  • Method for preparing double-layer membrane for spinal cord treatment

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Embodiment 1

[0036] 1) Make polylactic acid with a certain molecular weight (mass average molecular weight is 5000-1000000, preferably 5000-100000), dissolved in an organic solvent (organic solvents include 1,4-dioxane, acetone, chloroform / ethanol mixed Solvent, dichloromethane, tetrahydrofuran, and a mixed solvent of the above solvents), the concentration is 0.1-10 wt%, and the polylactic acid film is prepared by spin coating, drip coating, etc., as the outer sealing layer;

[0037] 2) The preparation process of making mesoporous bioactive glass is as follows. Add ethyl orthosilicate, calcium nitrate, F127, and 2M nitric acid into the mixture of absolute ethanol in a certain mass ratio and stir (the typical ratio is 2.7 g TEOS, 1.18 g Ca(NO3)2•4H2O, 4.2 g F127 and 0.08 g 2 M HNO3 was added to 8 g absolute ethanol), and stirred at room temperature for 3 h to obtain a sol. Put the obtained sol into a vacuum drying oven at 50°C for the sol-gel process. After the sol is gelled and dried, it ...

Embodiment 2

[0040] Compared with Example 1, the difference of Example 2 is that in 3), bioactive glass is used to load bioactive factors and mix with chitosan solution, and the mixed solution is covered on the polylactic acid film by spin coating or drop coating. .

[0041] 4) After the chitosan film is formed, the stem cell suspension is evenly immersed in the chitosan film in the previous step to culture the stem cells.

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Abstract

The invention provides a method for preparing a double-layer membrane for spinal cord treatment. A polylactic acid/bioactive glass/chitosan sandwich type composite membrane carrier supported with bioactive factors is established. The method comprises the following steps: 1) respectively preparing a polylactic acid membrane, mesoporous bioactive glass supported with bioactive factors and a chitosan membrane loaded with stem cells so as to serve as an outside sealing layer, a sandwich layer and an inside sustained-release and stem cell scaffold layer; and 2) covering the bioactive glass sandwich layer supported with bioactive factors on the polylactic acid membrane, covering the chitosan membrane loaded with stem cells on the bioactive glass layer so as to establish the sandwich composite membrane carrier. Therefore, the problems that in the prior art that a single damaged (but not dead) nerve tract cannot be repaired and treated and that targeted release of the medicine cannot be realized by the conventional drug delivery system and high concentrations of damaged local factors cannot be guaranteed are solved.

Description

technical field [0001] The invention relates to a method for making a double-layer film for spinal cord treatment. Background technique [0002] Currently, the most common route of administration of nerve growth-promoting or nerve regenerative drugs is intravenous or intrathecal injection. There are also gel-binding active factor patches applied on the surface of the spinal cord, and L-polylysine-binding active factor as a scaffold implanted in the spinal cord to solve the problem of factors passing through the blood-brain barrier. [0003] Both drug delivery systems and stem cell bioscaffolds can be used to treat spinal cord injuries, but the combination of the two has not been seen so far. A similar combination has been used in the method and material of stem cell three-dimensional scaffold for bridging nerves, such as (a composite repair material and its scaffold for bridging defective nerves CN201510716279.8). However, the current treatment methods for bridging nerve...

Claims

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Application Information

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IPC IPC(8): A61K9/70A61K38/18A61K35/51A61K35/28A61K47/36A61K47/34A61P25/00A61L27/40A61L27/18A61L27/20A61L27/38A61L27/54A61K9/51
CPCA61K9/0002A61K9/5146A61K9/703A61K35/28A61K35/51A61K38/18A61K38/1825A61K38/185A61K47/34A61K47/36A61L27/18A61L27/20A61L27/3834A61L27/54A61L2300/30A61L2300/412A61L2300/414A61L2300/624A61L2430/32C08L67/04C08L5/08
Inventor 顾军冯晓军范健纪立军宋晓丽徐林
Owner 无锡市锡山人民医院
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