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A method for extracting and displaying protein components in latent fingerprints

A protein and potential technology, applied in analytical materials, instruments, measuring devices, etc., can solve the problems of difficult extraction of protein components, difficulty in inspection and identification, and achieve the effect of avoiding non-specific adsorption problems, strong specificity and high sensitivity

Active Publication Date: 2019-11-08
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to make up for the deficiencies of the existing technology and solve the problems of difficult extraction of protein components and difficulties in later inspection and identification in the prior art, the present invention provides a method for extracting and displaying protein components in latent fingerprints

Method used

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  • A method for extracting and displaying protein components in latent fingerprints
  • A method for extracting and displaying protein components in latent fingerprints
  • A method for extracting and displaying protein components in latent fingerprints

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Extraction and visualization methods of protein components in latent fingerprints, including:

[0040] 1) Press the fingerprint on the non-porous glass substrate; the glass substrate is ultrasonically cleaned with absolute ethanol and water for 20 minutes;

[0041] 2) transfer the fingerprint sample on the substrate to 8% SDS-PAGE glue;

[0042] The 8% SDS-PAGE gel was prepared as follows:

[0043] Water: 4.6mL; 30% acrylamide: 2.7mL; 1.5M Tris (pH 8.8): 2.5mL; 10% SDS: 0.1mL; TEMED: 6μl;

[0044] 3) Use a wet transfer instrument, set the voltage to 120V, and transfer the time to 30 minutes, and transfer the target protein of the fingerprint sample on the SDS-PAGE gel to the PVDF membrane;

[0045] 4) After the transfer is completed, immerse the PVDF membrane in 5% (w / v) skim milk to seal for 1 hour;

[0046] 5) Wash the excess milk on the membrane with TBST, wash 3 times, 5 minutes each time;

[0047] 6) Add 0.02mg / mL primary antibody (rabbit anti-human EGF) dropwi...

Embodiment 2

[0056] Extraction and visualization methods of protein components in latent fingerprints, including:

[0057] 1) Press the fingerprint on the porous paper substrate (ordinary commercial A4 paper);

[0058] 2) transfer the fingerprint sample on the substrate to 10% SDS-PAGE glue;

[0059] A 10% SDS-PAGE gel was prepared as follows:

[0060] Water: 4.1mL; 30% acrylamide: 3.3mL; 1.5M Tris (pH 8.8): 2.5mL; 10% SDS: 0.1mL; TEMED: 6μl;

[0061] 3) Use a wet transfer instrument, set the voltage to 110V, and transfer for 30 minutes to transfer the target protein of the fingerprint sample on the SDS-PAGE gel to the PVDF membrane;

[0062] 4) After the transfer is completed, immerse the PVDF membrane in 4% (w / v) skim milk to seal for 1.5h;

[0063] 5) Wash the excess milk on the membrane with TBST, wash 3 times, 5 minutes each time;

[0064] 6) Add 0.03 mg / ml primary antibody (rabbit anti-human episomalin) dropwise to the PVDF membrane treated in step 6), and incubate the primary an...

Embodiment 3

[0071] Extraction and visualization methods of protein components in latent fingerprints, including:

[0072] 1) Press the fingerprint on the porous paper substrate (ordinary commercial A4 paper);

[0073] 2) transfer the fingerprint sample on the substrate to 10% SDS-PAGE glue;

[0074] A 10% SDS-PAGE gel was prepared as follows:

[0075] Water: 4.1mL; 30% acrylamide: 3.3mL; 1.5M Tris (pH 8.8): 2.5mL; 10% SDS: 0.1mL; TEMED: 6μl;

[0076] 3) Use a wet transfer instrument, set the voltage to 110V, and transfer for 30 minutes to transfer the target protein of the fingerprint sample on the SDS-PAGE gel to the PVDF membrane;

[0077] 4) After the transfer is completed, immerse the PVDF membrane in 4% (w / v) skim milk to seal for 1.5h;

[0078] 5) Wash the excess milk on the membrane with TBST, wash 3 times, 5 minutes each time;

[0079] 6) Add 0.03 mg / ml primary antibody (rabbit anti-human lysozyme) dropwise to the PVDF membrane treated in step 6), and incubate the primary anti...

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Abstract

The invention discloses an extraction and development method for protein ingredients in latent fingerprints, which belongs to the field of trace detection. Latent fingerprint samples remaining on different objects are transferred onto SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) gel; a wet transfer method is utilized to transfer target protein to a PVDF (polyvinylidene fluoride) membrane; a primary antibody capable of specifically identifying the target protein and a secondary antibody marked by horseradish peroxidase are chosen, and by utilizing the specific reaction between the antibodies and the target protein, the horseradish peroxidase is connected onto the target tested object; and chemiluminiscence is then utilized to develop the latent fingerprint. The extraction and development method can accurately extract protein ingredients in latent fingerprints, and can also keep the complete morphologies of fingerprints, i.e., the extraction and development method has the double functions of ingredient identification and fingerprint development. The extraction and development method has the characteristics of simplicity, high efficiency and easiness in operation, and has a good application prospect in the analysis of protein ingredients in latent fingerprints and the development of trace latent fingerprints.

Description

[0001] 1. Technical field [0002] The invention relates to the field of trace detection, in particular to a method for extracting and displaying protein components in latent fingerprints. [0003] 2. Background technology [0004] Fingerprint identification is one of the most reliable methods for personal identification. At present, fingerprint identification accounts for about 70% of the cases detected by the police using criminal technology. Fingerprints were once recognized by the judiciary as "the first physical evidence". Latent fingerprints contain complex components, such as endogenous proteins, amino acids, and drug metabolites, as well as exogenous explosives and prohibited drugs. These components can be identified and detected by corresponding antibodies. Fully and accurately understanding the composition of latent fingerprints will not only help people choose the appropriate display method to achieve the purpose of successfully identifying fingerprints, but also p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/535G01N33/68
Inventor 许林茹刘鄂湖吕志敏
Owner CHINA PHARM UNIV
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