Sugar polymer in cibotium barometz as well as preparation method and application of sugar polymer
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A golden retriever ridge and polymer technology, which is applied in application, food science, drug combination and other directions to achieve the effects of convenient operation, simple preparation process and remarkable effect
Inactive Publication Date: 2017-10-10
GUANGDONG PHARMA UNIV
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However, the research on the golden retriever ridge rough sugar polymer, refined sugar polymer and their prevention and treatment of osteoporosis has not been reported
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Embodiment 1
[0066] Embodiment 1, golden dog ridge sugar polymer
[0067] The golden dog ridge sugar polymer comprises golden dog ridge sugar polymer CBP-1 and golden dog ridge sugar polymer CBP-2;
[0068] The preparation method of described golden retriever ridge sugar polymer, comprises the following steps:
[0069] S1 cut into pieces: cut 10kg of the dried rhizome of golden retriever ridge into pieces to obtain the golden retriever ridge block, wash the above-mentioned golden retriever ridge block with water, and then soak for 12 hours to obtain the golden retriever ridge block after soaking;
[0070] S2 Water extraction: Carry out water extraction to the golden retriever dog ridge block obtained in step S1, the amount of water added during water extraction is 10 times the weight of the above golden dog ridge block, the water temperature is 85 ° C during water extraction, and the water extraction The time is 2 hours, the extract is collected, and the dregs are dried in the air;
[00...
Embodiment 2
[0078] Embodiment 2, the structure analysis of golden retriever dog ridge sugar polymer
[0079] (1) Monosaccharide composition analysis
[0080] Adopt PMP-column pre-column derivatization high-performance liquid chromatography to measure collection of patterns by complete acid hydrolyzate, the result is as follows figure 1 shown. figure 1 A is the spectrum of mixed monosaccharide standard products, in which 1-9 are mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose, respectively. figure 1 B is the monosaccharide composition map of CBP-1. The comparison shows that CBP-1 is composed of glucose and mannose.
[0081] (2) Infrared spectrum detection
[0082] The infrared spectrum detection results of CBP-1 are as follows: figure 2 As shown, the test results show that the characteristic absorption peak of CBP-1 containing sugar is: 3390.82cm -1 It is the characteristic absorption peak of O-H stretching vibration in sugar, ...
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Abstract
The invention discloses a sugar polymer in cibotium barometz as well as a preparation method and application of the sugar polymer. The preparation method comprises the step of carrying out grading-alcoholic precipitation on dried rhizome of cibotium barometz as a raw material by virtue of combination of a water-extraction grading-alcoholic precipitation method and an alkali-extraction alcoholic precipitation from a low ethanol concentration to a high ethanol concentration, so as to obtain cibotium barometz sugar polymers. A preparation process is simple and convenient in operation, and the large-scale production can be realized. The purified cibotium barometz sugar polymers are obtained by virtue of ion exchange column chromatography and molecular sieve gel column chromatography, and the two cibotium barometz sugar polymers CBP-1 and CBP-2 are prepared for the first time. The invention provides a preparation method of a raw sugar polymer and a refined sugar polymer in cibotium barometz and active research of the cibotium barometz sugar polymers in anti-osteoporosis, and the basis is provided for the application of the cibotium barometz sugar polymers in the fields of medicines, health products, foods and the like.
Description
technical field [0001] The invention belongs to the technical field of medicine and health food, and in particular relates to a golden-haired dog's ridge coarse sugar polymer (including oligosaccharides and polysaccharides), a refined sugar polymer (including oligosaccharides and polysaccharides) and a preparation method thereof, and also relates to a golden-haired dog's ridge sugar polymer (including oligosaccharides and polysaccharides) in the preparation of medicines or health food or functional food for preventing and / or treating osteoporosis. Background technique [0002] Osteoporosis is a bone degenerative disease characterized by decreased bone mineral mass, damaged bone microarchitecture, and decreased bone strength, resulting in increased bone fragility and susceptibility to fracture. With the prolongation of human life and the development of social aging, fractures caused by osteoporosis greatly increase the disability and mortality of the elderly, and at the same ...
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