A kind of SSR primer and method for purity identification of zucchini hybrid seeds

A technology for the purity and identification method of hybrid seeds, which is applied in the identification of the purity of zucchini hybrid seeds and the field of identified SSR primers, can solve the problems that have not been seen in identifying the purity of zucchini hybrid seeds, etc., and achieve high commercial application value, strong specificity, and low cost Effect

Active Publication Date: 2020-11-10
CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the identification of the purity of zucchini hybrid seeds using SSR molecular marker technology

Method used

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  • A kind of SSR primer and method for purity identification of zucchini hybrid seeds
  • A kind of SSR primer and method for purity identification of zucchini hybrid seeds
  • A kind of SSR primer and method for purity identification of zucchini hybrid seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Establishment of a method for detecting the purity of hybrid zucchini seeds

[0027] 1. Screen the SSR primers for purity identification

[0028] Several pairs of primers designed according to the sequencing results of the zucchini transcriptome were screened between parents and offspring, and the primer SGSSR25 with a co-dominant differential marker band was selected. The marker band pattern is clear and reproducible. The sequence is as follows:

[0029] SGSSR25-F: 5'- GGCATTTTCCTCCCCATTTAT-3' (SEQ ID NO. 1); SGSSR25-R: 5'-AAATATGTGGCCAGCGACTC-3' (SEQ ID NO. 2).

[0030] Using zucchini genomic DNA as a template, PCR amplification was performed using the above-mentioned SSR primer SGSSR25, and the amplified product was subjected to gel electrophoresis. The electrophoresis results showed that the SSR primer SGSSR25 could produce a 256bp paternal-specific band and a 212bp maternal-specific band Bands.

[0031] 2. Using the above-mentioned specific primers to i...

Embodiment 2

[0047] Adopt the method of embodiment 1 to 12 strains of zucchini that are taken from the seed purity identification field of vegetable base of Fujian Academy of Agricultural Sciences, number its single plant, extract single plant DNA and detect (see figure 2 ), test results: 11 hybrid seeds and 1 male parent, the seed purity was 91.67%, which was consistent with the field test results.

Embodiment 3

[0049] Adopt the method of embodiment 1 to 11 strains of zucchini that are taken from the seed purity identification field of vegetable center base, its single plant numbering, extracting single plant DNA detects (see image 3 ), test results: 10 hybrid seeds and 1 female parent, the seed purity was 90.9%, which was consistent with the field test results.

[0050] The above examples show that the method of the present invention can effectively distinguish the zucchini hybrid seeds from their parent seeds, and quickly and accurately detect the purity of the seeds.

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Abstract

The invention discloses an SSR primer for identifying the purity of hybrid seeds of summer squashes. The primer comprises an upstream primer SGSSR25-F and a downstream primer SGSSR25-R of a primer SGSSR25, wherein the upstream primer SGSSR25-F has a nucleotide sequence as shown in SEQ ID NO.1; and the downstream primer SGSSR25-R has a nucleotide sequence as shown in SEQ ID NO.2. The invention further discloses a method for identifying the purity of hybrid seeds of summer squashes. The primer and the method can be used for effectively differentiating hybrid seeds of summer squashes and female parent and male parent seeds thereof and quickly and accurately detecting the purity of hybrid seeds, are rapid and accurate, and have the advantages of low cost, simple operation and the like.

Description

technical field [0001] The invention belongs to the technical field of agricultural vegetable breeding and application, and in particular relates to an SSR primer for identifying the purity of zucchini hybrid seeds and a method for identifying the purity of zucchini hybrid seeds. Background technique [0002] squash( Cucubita pepo L. ) is also called American squash, which is an annual vine herb of Cucurbitaceae Cucurbita. Zucchini is one of the earliest crops cultivated by human beings. It is delicious, nutritious, and has medicinal value and is loved by the public. In the production of zucchini, its adaptability is strong. The planting cycle is short, the cultivation is relatively easy, the product yield is high, the supply cycle is very long, and it is also relatively easy to store and is suitable for intercropping. It has a wide range of planting in my country and has become one of the main varieties of melon vegetables in my country. First, the cultivated area is sec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 朱海生温庆放李永平王彬刘建汀陈敏氡林珲张前荣叶新如黄丽芳
Owner CROP RES INST OF FUJIAN ACAD OF AGRI SCI
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