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A method for distinguishing maize haploid

A haploid, maize technology, applied in the directions of botanical equipment and methods, instruments, plant genetic improvement, etc., can solve problems such as applicability limitations, and achieve the effect of simplifying the identification steps

Active Publication Date: 2019-11-01
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, these systems for sorting haploids based on visual recognition of color have limitations in applicability due to the need for clear color expression of the kernels

Method used

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  • A method for distinguishing maize haploid
  • A method for distinguishing maize haploid
  • A method for distinguishing maize haploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the preparation of hybrid population

[0046] 1. Preparation of DH series

[0047] 1. Maize inbred line Qi 319 (female parent) was crossed with corn inbred line Chang 7-2 (male parent) to obtain hybrid offspring (grain).

[0048] 2. The hybrid progeny (plant) obtained in step 1 is used as the female parent, and is crossed with the induced line CAU5 as the male parent to obtain the hybrid progeny (grain).

[0049] 3. Select pseudo-haploids from the hybrid offspring (grains) obtained in step 2, and perform haploid doubling to obtain 107 DH lines. Named successively as DH series 1 to DH series 107.

[0050] 2. Preparation of Hybrid Population A

[0051] Each DH line obtained in step 1 is used as the female parent, and the induced line CHOI3 is used as the male parent to perform hybridization to obtain hybrid offspring (grains). 30-40 pseudo-haploids and n1 (n1=10 or 0) pseudo-diploids were randomly selected from the hybrid offspring (grain) obtained from ...

Embodiment 2

[0063] Embodiment 2, establishing a model to identify haploids by collecting infrared spectra

[0064] 1. Grain near-infrared transmission spectrum acquisition

[0065] Each grain in the hybrid population A prepared in Example 1, the hybrid population B prepared in Example 1, and the hybrid population C prepared in Example 1 was respectively subjected to near-infrared transmission spectrum scanning (the embryo surface of the grain faces the light source, and the light source is 3 cm away from the spectrometer ). The spectrometer is a micro spectrometer MicroNIR1700 produced by JDSU Company, the spectral range is 950-1600nm, and the single measurement time is 1s. Micro spectrometer MicroNIR1700 collects near-infrared transmission spectrum absorption values ​​at 125 specific wavelengths in the spectral range 950-1600nm.

[0066] 2. Authenticity Identification of Pseudo-Haploid and Pseudo-Diploid

[0067] After step 1 is completed, each seed in the hybrid population A prepared...

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Abstract

The invention discloses a method for identifying maize haploids. The method for identifying maize haploids provided by the invention comprises the following steps: (1) taking whether each corn kernel in a training set is a real haploid or a real diploid as a dependent variable, taking a near-infrared transmittance spectroscopy absorption value of each corn kernel as an independent variable, and establishing a haploid identification model by using a machine learning algorithm, wherein the training set is composed of a plurality of corn kernels, one part of the corn kernels are the real haploids, and the other part of the corn kernels are the real diploids; and (2) taking to-be-detected corn kernels, acquiring the near-infrared transmittance spectroscopy absorption values, inputting the absorption values into the haploid identification model established in the step (1), and outputting the result that the to-be-detected corn kernels are predicted haploids or predicted diploids by the model. The method provided by the invention can be used for automatic identification and has significances for promoting the maize haploid breeding technique engineering.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for identifying haploids of maize. Background technique [0002] Corn is the crop with the largest planting area in my country, and it is also an important food crop, which plays an irreplaceable role in the fields of national production and food security. In 2006, the planting area of ​​corn was 26.66 million hectares. In 2009, it surpassed rice to become the crop with the largest planting area in my country. According to data from the website of the National Bureau of Statistics, in 2016, the national corn planting area was 36.76 million hectares, with a total output of 219 million tons, and the corn yield level reached 5972.7 kg / ha. The continuous improvement of the yield level of maize is inseparable from the genetic improvement of germplasm and the selection of excellent hybrids. The single-cross varieties widely used in production make maize a model of h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/08G01N21/359G16B25/00
CPCA01H1/02A01H1/08G01N21/359G16B25/00
Inventor 陈绍江李伟李金龙陈琛钟裕
Owner CHINA AGRI UNIV
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