A method for identifying maize haploid kernels based on NMR cpmg decay curves

An attenuation curve and haploid technology, applied in the field of corn haploid grain identification, can solve problems such as visual fatigue, differences in expression clarity, and differences in identification accuracy
CN108445035BActive Publication Date: 2021-02-02CHINA AGRI UNIV

Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-02-02

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Abstract

The invention discloses a method for identifying corn haploid grains based on nuclear magnetic resonance CPMG decay curve. The method provided by the invention includes the following steps: (1) collecting the nuclear magnetic signals of each corn kernel in the training set, and obtaining the normalized CPMG decay curve of the quality of each kernel; (2) performing data processing on the 0-600ms section, and then Carry out principal component analysis, and then construct a haploid identification model; (3) take the corn kernels to be tested, collect NMR signals, and obtain a mass-normalized CPMG decay curve; (4) perform data processing on the 0-600ms section, Then carry out principal component analysis, and then substitute the results into the haploid identification model, and output the results from the model. The method provided by the invention can be used for automatic identification and plays an important role in promoting the engineering of maize haploid breeding technology. The method for identifying maize haploids provided by the invention is simple, feasible, fast and efficient, has wide applicability, and has great application and popularization value.
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Description

technical field

[0001] The invention relates to the field of identification of haploid corn grains, in particular to a method for identifying haploid corn grains based on nuclear magnetic resonance CPMG decay curves. Background technique

[0002] Maize originates in Central and South America and has been introduced to China for more than 400 years. Due to its high yield, wide application, strong adaptability and rapid development of cultivated area, it has become the largest crop in my country. At the same time, corn is the crop with the highest degree of commercialization, and the commercial operation model requires seed companies to keep up with the trend of variety updates and quickly cultivate corn varieties that are suitable for the market. DH breeding technology can reduce line selection time, shorten breeding years and improve breeding efficiency.

[0003] Maize haploid technology is a set of easy-to-engineer breeding technology, which includes basic material prepara...

Claims

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