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A method for identifying maize haploid kernels based on NMR cpmg decay curves

An attenuation curve and haploid technology, applied in the field of corn haploid grain identification, can solve problems such as visual fatigue, differences in expression clarity, and differences in identification accuracy

Active Publication Date: 2021-02-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the R1-nj color marking system are: some germplasm materials contain dominant suppressor genes such as C1-I, and there are great differences in the expression clarity of R1-nj in the grains; Visual fatigue, and there are differences in identification accuracy among different personnel

Method used

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  • A method for identifying maize haploid kernels based on NMR cpmg decay curves
  • A method for identifying maize haploid kernels based on NMR cpmg decay curves
  • A method for identifying maize haploid kernels based on NMR cpmg decay curves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1, the preparation of hybrid population

[0088] The method of identifying haploid and heterozygous diploid: after the ears of corn are mature, harvest the ears obtained by hybridization and place them in a dry environment to dry; In the selection of individual (diploid) grains, those with purple endosperm and colorless embryo scutellum are haploid grains, and those with purple endosperm and purple embryo scutellum are heterozygous diploid grains. Using this method to screen haploids and heterozygous diploids from the hybrid progenies obtained in this example, the haploids are true haploids, and the heterozygous diploids are true diploids.

[0089] Time: 2017. Location: Hainan.

[0090] The maize Zhengdan 958 was used as the female parent, and the maize haploid inducible line CAU3 was used as the male parent to perform hybridization to obtain hybrid offspring (grains); 45 haploid and 45 hybrid offspring were randomly selected from the hybrid offspring (grai...

Embodiment 2

[0099] Embodiment 2, NMR signal acquisition

[0100] Each grain in the hybrid population B is processed as follows:

[0101] 1. Weighing.

[0102] 2. Using nuclear magnetic resonance instrument and supporting nuclear magnetic resonance analysis software "CPMG (carr-purcell-meiboom-gill, CPMG) pulse sequence" for nuclear magnetic signal acquisition. The parameters are set as follows: TW=800ms, TE=0.600ms, NS=16. Obtain a CPMG decay curve for each kernel.

[0103] 3. Quality normalization (eliminate the influence of grain weight on signal volume)

[0104] The amplitude corresponding to each time point was divided by the weight of the grain to normalize the data to obtain a mass-normalized CPMG decay curve (one mass-normalized CPMG decay curve was obtained for each grain).

[0105] The software used for mass normalization is: Microsoft Excel 2016 MSO 32-bit.

[0106] 4. Band selection

[0107] The mass-normalized CPMG decay curve (full relaxation time) of each grain is show...

Embodiment 3

[0111] Embodiment 3, the selection of data processing mode

[0112] The model was established 100 times, and the results were averaged. In each model establishment, 80% haploids and 80% heterozygous diploids are randomly selected from hybrid population B to form the training set, and the remaining 20% ​​haploids and 20% heterozygous diploids form the verification set.

[0113] 1. The data of the training set grains are processed as follows

[0114] Carry out data processing to the 0-600ms section of the CPMG decay curve after the quality normalization that embodiment 2 obtains, then carry out principal component analysis, adopt the first 100 principal components as variables, adopt support vector machine algorithm to construct haploid discrimination Model.

[0115] Data processing methods are: 10-point smoothing (S), first-order derivation (D), vector normalization (V), 10-point smoothing followed by first-order derivation (SD), 10-point smoothing Afterwards, vector normali...

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Abstract

The invention discloses a method for identifying corn haploid grains based on nuclear magnetic resonance CPMG decay curve. The method provided by the invention includes the following steps: (1) collecting the nuclear magnetic signals of each corn kernel in the training set, and obtaining the normalized CPMG decay curve of the quality of each kernel; (2) performing data processing on the 0-600ms section, and then Carry out principal component analysis, and then construct a haploid identification model; (3) take the corn kernels to be tested, collect NMR signals, and obtain a mass-normalized CPMG decay curve; (4) perform data processing on the 0-600ms section, Then carry out principal component analysis, and then substitute the results into the haploid identification model, and output the results from the model. The method provided by the invention can be used for automatic identification and plays an important role in promoting the engineering of maize haploid breeding technology. The method for identifying maize haploids provided by the invention is simple, feasible, fast and efficient, has wide applicability, and has great application and popularization value.

Description

technical field [0001] The invention relates to the field of identification of haploid corn grains, in particular to a method for identifying haploid corn grains based on nuclear magnetic resonance CPMG decay curves. Background technique [0002] Maize originates in Central and South America and has been introduced to China for more than 400 years. Due to its high yield, wide application, strong adaptability and rapid development of cultivated area, it has become the largest crop in my country. At the same time, corn is the crop with the highest degree of commercialization, and the commercial operation model requires seed companies to keep up with the trend of variety updates and quickly cultivate corn varieties that are suitable for the market. DH breeding technology can reduce line selection time, shorten breeding years and improve breeding efficiency. [0003] Maize haploid technology is a set of easy-to-engineer breeding technology, which includes basic material prepara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 陈绍江李金龙李伟焦炎炎张俊稳陈琛陈明刘晨旭田小龙钟裕祁晓龙王鼎昌
Owner CHINA AGRI UNIV
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