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A kind of lysine-Aureobasidium pullulans melanin biological hair dye and preparation method thereof

A technology of Aureobasidium pullulans and melanin, applied in the field of beauty and hairdressing, can solve problems such as inability to be used repeatedly, affect enzymatic efficiency, single enzyme, etc., and achieve strong coloring ability, stable properties, and good water solubility

Active Publication Date: 2020-06-16
广州健道健康产业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN1420144A extracts tyrosine oxidase from plants, uses tyrosine monohydric phenol derivatives as substrates, and controls enzymatic synthesis reaction conditions to synthesize melanin. The enzyme used is relatively simple, which affects the enzymatic efficiency and cannot be used repeatedly. In addition, the cost of extracting tyrosinase from plants is relatively high
[0005] The use of microorganisms to produce melanin, especially the use of melanin-producing Aureobasidium pullulans to prepare natural melanin, and thus to prepare hair dyes has not been reported

Method used

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  • A kind of lysine-Aureobasidium pullulans melanin biological hair dye and preparation method thereof
  • A kind of lysine-Aureobasidium pullulans melanin biological hair dye and preparation method thereof
  • A kind of lysine-Aureobasidium pullulans melanin biological hair dye and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A lysine-Aureobasidium pullulans melanin biological hair dye is prepared from the following raw materials in weight percentage: 10% lysine-Aureobasidium pullulans melanin, 5% lipid, 2% surfactant, mordant Agent 1%, γ-polyglutamic acid 10%, hair nutrient 0.5%, sodium benzoate 0.1%, and the balance is water.

[0038] Described, lysine-Aureobasidium pullulans melanin is prepared by the following steps:

[0039] 1) will Aureobasidium pullulans (Aureobasidium pullulans) Inoculate to shaker culture in potato liquid medium with 8% inoculum size;

[0040] The culture temperature is 28°C, the culture time is 96h, and the rotation speed of the shaker is 170 r / min;

[0041] The composition of the potato liquid medium is: 200 g / L of potatoes, 20 g / L of glucose, 1 L of distilled water, and pH 7.0.

[0042]2) Take 100mL of the cultured fermentation broth, acidify it with 1.0mol / L HCl for 4 hours, then add 48.9 mL of 60% ethanol solution, let it stand for 2 hours, and filter it wit...

Embodiment 2

[0052] A lysine-Aureobasidium pullulans melanin biological hair dye is prepared from the following raw materials in percentage by weight: 5% lysine-Aureobasidium pullulans melanin, 8% lipid, 6% surfactant, mordant Agent 0.5%, γ-polyglutamic acid 5%, hair nutrition 1.5%, sodium benzoate 0.2%, and the balance is water.

[0053] Described, lysine-Aureobasidium pullulans melanin is prepared by the following steps:

[0054] 1) will Aureobasidium pullulans (Aureobasidium pullulans) Inoculate it into the potato liquid medium with a 5% inoculum size and cultivate it on a shaker;

[0055] The culture temperature is 28°C, the culture time is 96h, and the rotation speed of the shaker is 170 r / min;

[0056] The composition of the potato liquid medium is: 200 g / L of potatoes, 20 g / L of glucose, 1 L of distilled water, and pH 7.0.

[0057] 2) Take 100mL of the cultured fermentation broth, acidify it with 1.0mol / L HCl for 4 hours, then add 48.9 mL of 60% ethanol solution, let it stand for...

Embodiment 3

[0070] A lysine-Aureobasidium pullulans melanin biological hair dye is prepared from the following raw materials in percentage by weight: 1% lysine-Aureobasidium pullulans melanin, 10% lipid, 10% surfactant, mordant Agent 0.1%, γ-polyglutamic acid 1%, hair nutrient 3%, sodium benzoate 0.3%, and the balance is water.

[0071] Described, lysine-Aureobasidium pullulans melanin is prepared by the following steps:

[0072] 1) will Aureobasidium pullulans (Aureobasidium pullulans) Inoculate it into the potato liquid medium with a 3% inoculum size and cultivate it on a shaker;

[0073] The culture temperature is 28°C, the culture time is 96h, and the rotation speed of the shaker is 170 r / min;

[0074] The composition of the potato liquid medium is: 200 g / L of potatoes, 20 g / L of glucose, 1 L of distilled water, and pH 7.0.

[0075] 2) Take 100mL of the cultured fermentation broth, acidify it with 1.0mol / L HCl for 4 hours, then add 48.9 mL of 60% ethanol solution, let it stand for ...

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Abstract

The invention discloses a lysine-aureobasidium pullulans melanin biological tint which is prepared from the following raw materials in percentage by weight: 1-10% of lysine-aureobasidium pullulans melanin, 5-10% of lipid, 2-10% of a surfactant, 0.1-1% of a mordant, 1-10% of gamma-polyglutamic acid, 0.5-3% of a hair nutrient, 0.1-0.3% of a preservative and the balance of water. The invention further discloses a preparation method of the biological tint. Due to modification of the lysine, the aureobasidium pullulans melanin has the property of water solubility, so that the lysine-aureobasidium pullulans melanin is of a chemical structure similar to that of human hair melanin and is stable in property in environments such as sunshine, ultraviolet light, high temperature and strong oxidants, and thus the prepared biological tint has the characteristics of being good in water solubility, free of toxic or side effect, natural in color and long-lasting in color, and in addition is low in cost, free of hair damage and stable in dyeing effect.

Description

technical field [0001] The invention relates to the field of beauty and hairdressing, in particular to a lysine-Aureobasidium pullulans melanin biological hair dye and a preparation method thereof. Background technique [0002] With the improvement of people's living standards, hair dye, as a daily beauty cosmetic that changes hair color, has gradually entered the homes of ordinary people, and has become an embellishment for modern people's self-expression and pursuit of individuality. On the one hand, hair dye can make hair color return to nature. , to make up for some congenital deficiencies or acquired regrets, such as orientals traditionally regard natural black hair as beauty, once gray hair is caused by factors such as genetics, metabolism, nutrition or disease, old age, etc., people need to modify it with hair dye, so that On the other hand, as an embellishment of personality and fashion, many people request to change their single hair color, and use hair dye to chang...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61K8/9728A61K8/92A61K8/88A61K8/46A61K8/37A61K8/19A61Q5/10
CPCA61K8/19A61K8/375A61K8/463A61K8/64A61K8/88A61K8/925A61K2800/43A61K2800/524A61K2800/82A61Q5/10
Inventor 杨革李敏车程川刘金锋巩志金
Owner 广州健道健康产业科技有限公司
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