Method for determining absolute abundance of microbial community structure in environment

Abstract

The invention discloses a method for determining absolute abundance of a microbial community structure in an environment. The method comprises the following steps: extracting DNA in an environmental sample, obtaining a DNA sample, and determining a total concentration of classification genes in the DNA sample through a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method; obtaining the relative abundance of the classification genes in microbes of different kingdoms, phyla, classes, orders, families, categories and species through a second-generation high-throughput sequencing method; and finally, multiplying the total concentration of the classification genes in the DNA sample by the relative abundance of the classification genes in the microbes of different kingdoms, phyla, classes, orders, families, categories and species, and calculating to obtain the absolute abundance of the classification genes in the microbes of different kingdoms, phyla, classes, orders, families, categories and species, so that rapid high-throughput determination of the absolute abundance of the microbial community structure is realized. Therefore, cross-group sample comparison is reliable, growth and decline of the species can be substantially reflected, and a technical support is provided for further exploration of the microbial community structure.

Description

technical field [0001] The invention relates to the field of microbial ecology, in particular to a method for determining the absolute abundance of microbial community structures in the environment. Background technique [0002] Microbes are an important part of the ecological environment system and play a vital role in the earth's material cycle and energy flow. Because of their rich species diversity, gene diversity and ecosystem diversity, they have great potential for mining , has attracted the attention of researchers. It is estimated that the number of microorganisms in the earth is about 4-6×10 30 cells, up to 10 species 6 kind. [0003] The study of microbial community structure is the basis of environmental microbial ecology. In the past hundred years, scientists have conducted extensive research on the environmental microbial community structure, such as plate counting, microscope observation, phospholipid fatty acid analysis, and denaturing gradient gels. Elec...

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Problems solved by technology

However, the two have different functions for describing a single species. Absolute abundance can be used to describe the absolute content of a single species, representing its increase or decrease in different or the same environmental communities; relative abundance is mainly used to describe the proportion of a single species in the entire Percentage of environmental communities, describing the degree of dominance of a single species, but cannot be used for comparison across samples

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