Preservation solution for fresh amnion, preservation method for fresh amnion and application of fresh amnion

A preservation method and preservation solution technology, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of high loss rate of active factors, increased chances of virus transmission, and inability to store at room temperature, etc., and achieve the convenience of use Effect

Active Publication Date: 2017-10-27
JIANGXI RUIJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has the following shortcomings: serum components are added to the preservation solution, which increases the chance of virus transmission between the amnion and the preservation solution; it only slightly prolongs the preservation time of the amnion; it needs to be stored at 4°C and cannot be stored at room temperature; Contains a high loss rate of active factors

Method used

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  • Preservation solution for fresh amnion, preservation method for fresh amnion and application of fresh amnion
  • Preservation solution for fresh amnion, preservation method for fresh amnion and application of fresh amnion
  • Preservation solution for fresh amnion, preservation method for fresh amnion and application of fresh amnion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A fresh amniotic membrane preservation solution, calculated as 1000ml preservation solution, consists of the following components: DMEM medium 42g, chondroitin sulfate 24g, low molecular weight dextran 16g, sodium pyruvate 8ml, non-essential amino acid 15ml, gibberellin 8ml, HEPES 16ml , pentaerythritol 13g, ammonium chloride 2g, DMPU 4ml, nano silicon dioxide 18g, carotene 9ml, indole acetic acid 13ml, calcium cyanamide 6g, hydrocortisone 4g, the balance is water for injection; the pH value is 7.2~ 7.4, the osmotic pressure is 350-380mOsm / L.

[0023] Preparation method: 42g DMEM medium, 24g chondroitin sulfate, 16g low molecular weight dextran, 8ml sodium pyruvate, 15ml non-essential amino acid, 8ml gibberellin, 16ml HEPES, 13g pentaerythritol, 2g ammonium chloride, 4ml DMPU, 18g nano Add silicon dioxide, 9ml carotene, 13ml indole acetic acid, 6g calcium cyanamide, 4g hydrocortisone to 500ml water for injection and mix well, then continue to add water for injection to ...

Embodiment 2

[0026] A fresh amniotic membrane preservation solution, calculated as 1000ml preservation solution, consists of the following components: DMEM medium 30g, chondroitin sulfate 20g, low molecular weight dextran 10g, sodium pyruvate 5ml, non-essential amino acid 10ml, gibberellin 5ml, HEPES 10ml , pentaerythritol 10g, ammonium chloride 2g, DMPU 3ml, nano silicon dioxide 15g, carotene 8ml, indole acetic acid 12ml, calcium cyanamide 5g, hydrocortisone 3g, the balance is water for injection; the pH value is 7.2~ 7.4, the osmotic pressure is 350-380mOsm / L.

[0027] Preparation method: 30g DMEM medium, 20g chondroitin sulfate, 10g low molecular weight dextran, 5ml sodium pyruvate, 10ml non-essential amino acid, 5ml gibberellin, 10ml HEPES, 10g pentaerythritol, 2g ammonium chloride, 3ml DMPU, 15g nano Add silicon dioxide, 8ml carotene, 12ml indole acetic acid, 5g calcium cyanamide, 3g hydrocortisone to 500ml water for injection and mix well, then continue to add water for injection to ...

Embodiment 3

[0030] A fresh amniotic membrane preservation solution, calculated as 1000ml preservation solution, consists of the following components: DMEM medium 50g, chondroitin sulfate 30g, low molecular weight dextran 20g, sodium pyruvate 10ml, non-essential amino acid 20ml, gibberellin 10ml, HEPES 20ml , pentaerythritol 20g, ammonium chloride 3g, DMPU 5ml, nano silicon dioxide 20g, carotene 10ml, indole acetic acid 15ml, calcium cyanamide 6g, hydrocortisone 5g, the balance is water for injection; the pH value is 7.2~ 7.4, the osmotic pressure is 350-380mOsm / L.

[0031]Preparation method: 50g DMEM medium, 30g chondroitin sulfate, 20g low molecular weight dextran, 10ml sodium pyruvate, 20ml non-essential amino acids, 10ml gibberellin, 20ml HEPES, 20g pentaerythritol, 3g ammonium chloride, 5ml DMPU, 20g nano Add silicon dioxide, 10ml carotene, 15ml indole acetic acid, 6g calcium cyanamide, 5g hydrocortisone to 500ml water for injection and mix well, then continue to add water for injecti...

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PUM

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Abstract

The invention provides a preservation solution for a fresh amnion. The preservation solution per 1,000ml is prepared from the following ingredients: 30g to 50g of DMEM culture medium, 20g to 30g of chondroitin sulfate, 10g to 20g of low molecular dextran, 5ml to 10ml of sodium pyruvate, 10ml to 20ml of non-essential amino acids, 5ml to 10ml of gibberellin, 10ml to 20ml of HEPES, 10g to 20g of pentaerythritol, 2g to 3g of ammonium chloride, 3ml to 5ml of DMPU, 15g to 20g of nano-silica, 8ml to 10ml of carotene, 12ml to 15ml of indoleacetic acid, 5g to 6g of calcium cyanamide, 3g to 5g of hydrocortisone and the balance of water for injection. By using the preservation solution provided by the invention, the fresh amnion can be preserved for 1 to 2 weeks at normal temperature and can be preserved for 1 year or more at the temperature of 4 DEG C, and the use is convenient; the form of the amnion is not changed, and the survival rate of cell active factors contained in the amnion is relatively high.

Description

technical field [0001] The invention relates to a fresh amniotic membrane preservation solution, in particular to a fresh amniotic membrane preservation solution and a fresh amniotic membrane preservation method and application. Background technique [0002] Amniotic membrane (thickness about 0.02 ~ 0.05mm) is derived from the embryo, is a transparent, no nerve, blood vessels and lymphatic vessels, low antigenicity and tough tissue, composed of epithelial cell layer, basement membrane, stroma layer, fibroblast layer , Sponge layer composition. Amniotic membrane plays an important role in many fields of ophthalmology, such as promoting epithelial repair, healing and differentiation, inhibiting inflammation, anti-angiogenesis, inhibiting fibrosis and scarring, and preventing synechia. With the increasing amount of amniotic membrane, the preservation of amniotic membrane, especially the preservation of the active ingredients of amniotic membrane, has become a hot spot in the f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 吕虎傅筱冲刘德武苗春云辛红周星滕志强
Owner JIANGXI RUIJI BIOTECH CO LTD
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