A compound micro-ecological preparation for degrading nitrite in aquaculture ponds and its application

A technology of compound microecology and preparation, applied in the field of aquaculture, can solve the problems of nitrite accumulation and bacterial diseases

Active Publication Date: 2020-07-07
无锡中水渔药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a compound micro-ecological preparation that degrades nitrite in aquaculture ponds, which has the effect of inhibiting Vibrio, so as to solve the problem of successive occurrence of nitrite accumulation and bacterial diseases

Method used

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  • A compound micro-ecological preparation for degrading nitrite in aquaculture ponds and its application
  • A compound micro-ecological preparation for degrading nitrite in aquaculture ponds and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1: Preparation of Pseudomonas Fredericksburg Powder

[0033] (1) Seed tank culture: prepare a sterile triangular flask with glass beads inside, scrape and wash the mature bacterial slurry on the inclined surface of the eggplant bottle with sterile water, put it into the triangular flask, and shake and disperse the bacterial slurry to obtain a uniform bacterial suspension. 2%-10% by volume of the inoculum into the 100L seed tank, the filling coefficient of the seed tank is 60%-70% (v / v), that is, 60-70L of 100L seed canning medium.

[0034] The cultivation conditions of the seed tank are as follows: the stirring speed is 200r / min, the ventilation rate is 1-3m3 / h, the cultivation temperature is 25℃, and the cultivation time is 20-48h.

[0035] Seed tank medium: glucose 6g / L, yeast powder 12g / L, MgSO4.7H2O 3.5g / L, KH2PO4 2.5g / L, CaCl2 1.5g / L, pH7.0-7.2.

[0036] (2) Fermentation culture: the seed culture liquid is connected to the 1T fermenter with the inoculum vo...

Embodiment 2

[0048] Embodiment 2: the preparation of bacillus subtilis powder

[0049] (1) Strain activation: Aseptically open the freeze-dried and preserved strain of Bacillus subtilis, streak and inoculate it on the slant of nutrient agar test tube, cultivate it at 30-37°C for 24-48h, and then transfer it to the slant of nutrient agar eggplant bottle by streaking , Culture at 30-37°C for 24-48h; microscopic examination, when more than 90% of the bacteria form spores, it is mature.

[0050] Slant medium composition in g / L: peptone 10, beef extract 3, NaCl 5, agar 15-20, prepared with distilled water to constant volume, pH 7.0-7.2;

[0051] Seed tank cultivation: Prepare a sterile triangular flask with glass beads inside, scrape and wash the mature fungus sludge on the slope of the eggplant bottle with sterile water, put it into the triangular flask, oscillate to disperse the fungus slime, and obtain a uniform bacterial suspension. Heat the bacterial suspension in a water bath at 80°C for...

Embodiment 3

[0056] Embodiment 3: the preparation of bacillus amyloliquefaciens powder

[0057] (1) Strain activation: Aseptically open the freeze-dried and preserved strain of Bacillus amyloliquefaciens JSSW-LA, streak and inoculate it on the slant of nutrient agar test tube, culture it at 30-37°C for 24-48h, and then transfer the streak to the nutrient On the slant of agar eggplant bottle, culture at 30-37°C for 24-48h; microscopic examination, when more than 90% of the bacteria form spores, it is mature.

[0058] Slant medium composition in g / L: peptone 10, beef extract 3, NaCl 5, agar 15-20, prepared with distilled water to constant volume, pH 7.0-7.2;

[0059] (2) Seed tank cultivation: Prepare a sterile triangular flask with glass beads inside, scrape and wash the mature fungal slime on the slope of the eggplant bottle with sterile water, put it into the triangular flask, oscillate and disperse the fungal slime, and obtain a uniform bacterial suspension liquid. Heat the bacterial s...

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Abstract

The invention discloses a compound micro-ecological preparation for degrading nitrite in a culture pond and application. The invention discloses a microorganism preparation for degrading the nitrite in a shrimp pond and inhibiting pathogenic bacteria, in particular relates to the compound micro-ecological preparation for degrading the nitrite, and belongs to the technical field of culture. The micro-ecological preparation, which contains mixed powder and small granules, is prepared by selecting pseudomonas, bacillus amyloliquefaciens and bacillus subtilis which have a denitrification effect, and taking wheat bran, zeolite powder and corn starch as auxiliary materials. According to the compound micro-ecological preparation disclosed by the invention, on one hand, a small molecular carbon source is introduced into a water body, so that the metabolic activity of original denitrifying bacteria and heterotrophic nitrification bacteria of the pond is enhanced; on the other hand, microorganism bacteria play a role on a pond bottom and a water body at the same time through adsorbents with different specific gravities, so that the release of ammonia nitrogen and the nitrite at the pond bottom is reduced; thirdly, the bacillus amyloliquefaciens is utilized, so that the quantity of pathogenic bacteria including vibrio and the like can be inhibited while the water quality is improved, and furthermore, fish diseases are reduced.

Description

technical field [0001] The invention relates to a microbial preparation for degrading nitrite in shrimp ponds and inhibiting pathogenic bacteria, in particular to a microbial preparation for degrading nitrite, and belongs to the technical field of aquaculture. Background technique [0002] At present, with the continuous development of aquaculture, the scale of high-density aquaculture continues to expand, and water pollution is becoming more and more serious. Among them, ammonia nitrogen and nitrite are too high, which not only directly harms the animals, but also causes a large number of pathogenic bacteria due to the deteriorating water environment. reproduce, leading to disease outbreaks. [0003] Microbial agents are widely recognized for reducing ammonia nitrogen and nitrite in water bodies. At present, many compound microbial preparations have appeared, but the use effect is not stable. Compared with nitrosating bacteria, autotrophic nitrifying bacteria have a slowe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34A01N63/22A01N63/27A01P1/00C12R1/38C12R1/07C12R1/125C02F101/16C02F103/20
CPCA01N63/00C02F3/341C02F2101/16C02F2101/166C02F2103/20C12N1/20
Inventor 宋萍夏冬张维娜施大林高亮匡群钱进王利风顾翔沈建夏燚
Owner 无锡中水渔药有限公司
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