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A strain of Yarrowia lipolytica and its application

A technology of Yarrowia and strains, applied in the field of biotechnology microorganisms, can solve the problems of low fruit content, seasonal restrictions, high cost and the like

Active Publication Date: 2020-08-28
浙江诺睿特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extremely low content in the fruit, the way of natural extraction has disadvantages such as high cost and seasonal restrictions

Method used

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  • A strain of Yarrowia lipolytica and its application
  • A strain of Yarrowia lipolytica and its application
  • A strain of Yarrowia lipolytica and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Cultivation of Yarrowia lipolytica (Yarrowia lipolytica) NYL01 strain.

[0028] 1 medium

[0029] 1.1 YPD medium: glucose 2%, peptone 2%, yeast powder 1%, water 1L, pH 5.0-5.5. Add 2% agar powder to the solid medium on this basis, and adjust the pH to 6.0-6.5.

[0030] 1.2 Transformation medium: glucose 2%, yeast powder 1%, ricinoleic acid 10%, potassium dihydrogen phosphate 0.05, disodium hydrogen phosphate 0.03%, magnesium sulfate 0.01%, Tween 0.4%, water 1L. pH natural.

[0031] 2 Experimental methods

[0032] 2.1 Isolation, purification, screening and mutagenesis of high-yielding γ-decalactone strains

[0033] Step 1: Weigh an appropriate amount of soil samples from different soil samples, put them into a shaker bottle filled with sterile water, oscillate and shake for a while, take a small amount of soil leaching solution, dilute it with sterile water to different concentrations, and draw 50uL Spread on YPD solid medium. Incubate at 28°C for 2 days....

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Abstract

The invention belongs to the field of biotechnology microorganisms, and in particular relates to a strain of Yarrowialipolytica (named NYL01) that biotransforms and produces γ-decalactone and its application. The strain is preserved in the China Center for Type Culture Collection (CCTCC), the preservation address: Wuhan University, Wuhan, China, the preservation number is: CCTCC NO: M2015696, and the preservation date is November 24, 2015. The strain has excellent characteristics such as high production of natural gamma-decalactone, high substrate conversion rate, simple culture conditions and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology microorganisms, in particular to a strain of Yarrowialipolytica (named as NYL01) for producing natural chiral gamma-decalactone by biotransformation and its application. Background technique [0002] γ-Decalactone is a ten-carbon compound containing a five-membered lactone ring. It has a strong peach and coconut aroma. It exists in a variety of fruits and dairy products. It is an internationally recognized safe food additive and pharmaceutical additive. Because of its attractive fragrance and low aroma threshold, it has been widely used in the fragrance industry. At present, γ-decalactone is mainly extracted from fruits or chemically synthesized. Due to the extremely low content in the fruit, the natural extraction method has disadvantages such as high cost and seasonal restrictions. The regulations on food additives and pharmaceutical additives strictly restrict the use of chemically synthesized pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P17/04C12R1/645
CPCC12P17/04C12N1/145C12R2001/645
Inventor 冯贻安沈情佳杨霄周俊辰
Owner 浙江诺睿特生物科技有限公司