Novel water soluble composite immune adjuvant and porcine circovirus disease vaccine
A water-soluble compound, immune adjuvant technology, applied in vaccines, viruses, antiviral agents, etc., can solve problems such as high cost
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[0035] Part 1 Adjuvant development and its immune adjuvant effect on PCV2 inactivated antigen
[0036] In this study, based on carbomer slow-release polymers, four aqueous adjuvants, CP974S, CP934S, CP974M and CP were configured, and inactivated PCV2 was used to prepare PCV2 inactivated vaccine, and the immune tests were carried out in mice and pigs. The results showed that , CP974S and CP934S two water-based adjuvants have a strong immune adjuvant effect on PCV2 inactivated antigens, and the immune effect of CP974S water-based adjuvant vaccine in pigs is better than that of PCV2ISA206 adjuvanted inactivated vaccine, which has important application prospects.
[0037] 1 Materials and methods
[0038] 1.1 Main materials
[0039] The PCV2SH strain virus liquid was constructed and preserved by our laboratory (the same kind of conventional strains are also applicable). PCV2 monoclonal antibody 3E5 was prepared and stored in our laboratory (similar conventional antibodies are also ...
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