Novel water soluble composite immune adjuvant and porcine circovirus disease vaccine

A water-soluble compound, immune adjuvant technology, applied in vaccines, viruses, antiviral agents, etc., can solve problems such as high cost

Active Publication Date: 2017-11-24
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

my country has developed 5 kinds of whole-virus inactivated vaccines and 2 kinds of subunit vaccines, and realized commercial production, but the adjuvants used are all oil-based...

Method used

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  • Novel water soluble composite immune adjuvant and porcine circovirus disease vaccine
  • Novel water soluble composite immune adjuvant and porcine circovirus disease vaccine
  • Novel water soluble composite immune adjuvant and porcine circovirus disease vaccine

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Experimental program
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Effect test

Embodiment Construction

[0035] Part 1 Adjuvant development and its immune adjuvant effect on PCV2 inactivated antigen

[0036] In this study, based on carbomer slow-release polymers, four aqueous adjuvants, CP974S, CP934S, CP974M and CP were configured, and inactivated PCV2 was used to prepare PCV2 inactivated vaccine, and the immune tests were carried out in mice and pigs. The results showed that , CP974S and CP934S two water-based adjuvants have a strong immune adjuvant effect on PCV2 inactivated antigens, and the immune effect of CP974S water-based adjuvant vaccine in pigs is better than that of PCV2ISA206 adjuvanted inactivated vaccine, which has important application prospects.

[0037] 1 Materials and methods

[0038] 1.1 Main materials

[0039] The PCV2SH strain virus liquid was constructed and preserved by our laboratory (the same kind of conventional strains are also applicable). PCV2 monoclonal antibody 3E5 was prepared and stored in our laboratory (similar conventional antibodies are also ...

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Abstract

The invention discloses a novel water soluble composite immune adjuvant and a porcine circovirus disease vaccine. Four aqueous adjuvants (CP974S, CP934S, CP974M, and CP) are screened and designed to prepare a PCV2 inactivated vaccine. The results of mouse immunity tests and pig immunity tests show that CP974S and CP934S have a strong immune adjuvant effect on the PCV2 inactivated vaccine, and the pig immunity protective effect of CP974S aqueous adjuvant is better than the PCV2 imported ISA206 adjuvant inactivated vaccine. A baculovirus expressed PCV2Cap recombinant protein is taken as the basis to prepare different adjuvant vaccines. The results of mouse immunity tests and pig immunity tests show that two aqueous adjuvants (CP974S and CP934S) also have a strong immunity adjuvant effect on the PCV2Cap recombinant protein and the pig immunity effect of CP974S adjuvant vaccine is better than imported PCV2Cap subunit vaccine. The CP974S aqueous adjuvant fills the gap of water soluble adjuvant for pigs in China. The developed PCV2 vaccine is safe and effect and has an important application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of biological immunity and specifically designs a new water-soluble compound immune adjuvant and porcine circovirus disease vaccine. Background technique: [0002] Immune adjuvant (immunologic adjuvant) is an important component of the preparation of protein inactivated vaccines, which can significantly enhance the weak antigenic substances such as polysaccharides or polypeptides to induce the body to produce specific immune responses; or use the least amount of antigen and the least number of vaccinations Stimulating the body can produce sufficient immune response and high-titer antibody, which can be maintained for a long time and exert a lasting effect. The mechanism by which adjuvants enhance immune responses is very complex and has not yet been fully understood. The functions of adjuvants mainly include: ① change the normal immune function, attract a large number of antigen-presenting cells to process...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/12A61P31/20
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/552A61K2039/55505A61K2039/55511A61K2039/55583A61K2039/55594A61K2039/575C12N2750/10034
Inventor 姜平白娟朱雪蛟王先炜
Owner NANJING AGRICULTURAL UNIVERSITY
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