Gibberella nt-1 and its application

A technology of gibberella and bacterial suspension, applied in the field of microorganisms, can solve the problems of inability to grow, poor tolerance, etc., and achieve the effect of wide application potential

Active Publication Date: 2020-06-02
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some studies have isolated and identified some strains with certain tolerance and adsorption properties to heavy metal copper, most of these strains have poor tolerance to acidity and cannot grow in orchard soils that are co-contaminated by acidification and heavy metals.

Method used

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  • Gibberella nt-1 and its application
  • Gibberella nt-1 and its application
  • Gibberella nt-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Isolation and identification of copper-resistant bacterial strain NT-1

[0023] (1) Isolation and screening of copper-resistant bacterial strain NT-1

[0024] The bacterial strain NT-1 of the present invention is isolated and screened from orchard soil where apples have been planted for a long time and copper-containing pesticide Bordeaux mixture has been sprayed. The separated media were basal medium (potato dextrose medium) and copper selective medium (CuSO 4 ·5H 2 O into Cu 2+ The concentration is 10g L -1 Add the stock solution of beef extract peptone basal medium and potato dextrose basal medium respectively to adjust to the required copper concentration). The solid medium is to add 20g L to the liquid medium -1 agar.

[0025] Separation and screening steps: take 5g of fresh soil samples and shake them in a conical flask filled with 50mL of sterile water for 20min to make a soil extract. The extract was diluted stepwise to 10 -3 、10 -4 、10 -...

Embodiment 2

[0032] Embodiment 2: the mensuration of growth curve of copper-resistant bacterial strain NT-1

[0033] Using the dry weight method, draw 1 mL of fresh strain seed liquid, inoculate it in a conical flask containing 50 mL of potato dextrose liquid culture liquid, cultivate at a constant temperature of 28 ° C and 150 rpm, and take out 3 parallel samples every 8 hours for suction filtration. The mycelia were dried in an oven at 80°C to a constant weight and weighed to draw a growth curve.

[0034] The growth curve of strain NT-1 is as follows Figure 4 shown. Under the conditions of 28°C and 150rpm, NT-1 entered the logarithmic growth phase 8 hours after inoculation, and entered the stationary phase after 96 hours.

Embodiment 3

[0035] Example 3: Tolerance characteristics and physiological responses of bacterial strain NT-1 to different concentrations of copper stress

[0036] Pick a ring of NT-1 mycelium and inoculate it on the Cu-containing 2+ Concentrations are 100, 200, 300, 400, 500 and 600mg L -1 The potato dextrose solid medium was placed in a constant incubator at 28°C for 5 days, the growth of mycelium on the solid plate was observed, and the diameter of the colony was measured and recorded.

[0037] The seed solution of strain NT-1 was inoculated in Cu-containing 2+ Concentrations are 100, 200, 300, 400, 500 and 600mg L -1 In the potato glucose liquid culture medium, culture in a constant temperature shaker at 28°C and 150rpm for 5 days, observe the growth of the strain in the liquid medium, centrifuge the strain and dry it at 80°C to constant weight, measure and record Mycelial biomass dry weight.

[0038] It can be seen from Table 1 that when NT-1 was cultured for 5 days, the 2+ Conce...

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Abstract

The invention belongs to the technical field of microorganisms, in particular to a kind of Gibberella sp.NT-1 and its 2+ Applications in Adsorption Removal. Gibberella sp.NT‑1 was deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms on April 17, 2017, with a preservation number of CGMCC No.13888. The bacteria has strong acid-resistance, copper-resistance and copper adsorption capacity. 2+ The concentration is 200mg L ‑1 , within 5d for Cu in solution 2+ The adsorption removal rate can reach 45.6%. The strain has a good application prospect in acidification and bioremediation of heavy metal-copper-contaminated soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a Gibberella sp. NT-1 and its 2+ Applications in Adsorption Removal. Background technique [0002] In fruit forestry production, due to the extensive use of copper-containing fungicides such as Bordeaux mixture, a large amount of copper is accumulated in the orchard soil, and the inappropriate application of fertilizers and pesticides and the acidification of the orchard soil caused by planting fruit trees for many years will further increase the amount of copper in the soil. Bioavailability increases the risk of fruit trees absorbing and enriching copper, reduces the yield and quality of fruit, and threatens human health. The primary problem to be solved in the microbial remediation technology of acidified and copper-contaminated soil in orchards is to screen microbial strains with high tolerance and adsorption capacity for complex pollution. Although some studies have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14B09C1/10C12R1/645
CPCB09C1/105C12N1/145C12R2001/645
Inventor 涂晨刘颖骆永明
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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