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Microscopic multi-focal-length image sequence processing method for 3D shape reconstruction

An image sequence, three-dimensional shape technology, applied in image data processing, image enhancement, image analysis and other directions, can solve the problems of displacement deviation, large noise, and many holes, and achieve the goal of eliminating holes and noise, and eliminating image translation and scale changes. Effect

Active Publication Date: 2017-11-24
NINGBO YONGXIN OPTICS
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a microscopic multi-focus image sequence processing method for three-dimensional shape reconstruction in view of the above-mentioned prior art. Problems with displacement deviation, excessive noise and too many holes

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0022] The invention provides a microscopic multi-focus image sequence processing method for three-dimensional shape reconstruction. Its overall block diagram is as figure 1 shown, including the following steps:

[0023] Step (1), first determine the highest point position and the lowest point position of the observation sample through the Z-axis focusing of the microscope, after determining the highest point position and the lowest point position of the observation sample, adjust the Z-axis focal length, and the highest point or the lowest point position of the observed object Adjust the lowest point to a clear position, then rotate the Z-axis wheel of the microscope according to a predetermined distance interval, and collect images of the observed sample accordingly until the lowest or highest point of the observed sample is clearly display...

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Abstract

The invention relates to a microscopic multi-focal-length image sequence processing method for 3D shape reconstruction. The method is characterized by comprising a step of focusing through a microscope Z axis and determining an upper surface position and a lower surface position of an observation object, adjusting a Z axis focal length, collecting one image with a certain interval, and obtaining a microscopic multi-focal-length image sequence, a step of converting each image into a gray image and detecting an average displacement through a phase correlation method, a step of judging whether the image registration of the whole image sequence is needed, a step of filtering the microscopic multi-focal-length image sequence, and a step of finding a clear point corresponding to each position in the image sequence, finding a point with a largest definition, recording a serial number of the image where the point is located in the sequence as height reference information, constructing a 3D information map, and using a median filter for filtering. The method has the advantage that 3D shape reconstruction has a displacement error in a traditional microscopic multi-focal-length image sequence processing method, the noise is too large and cavities are excessive is solved.

Description

technical field [0001] The invention belongs to the field of microscopic image processing, in particular to a microscopic multi-focus image sequence processing method for three-dimensional shape reconstruction. Background technique [0002] The three-dimensional information of objects under the microscope has a wide range of application requirements in the fields of life medicine and industry, and is one of the core functions necessary for modern high-end digital microscopes. Whether it is a metallographic microscope or a biological microscope, since the depth of field of the microscope is very small, and the surface of the sample has a certain thickness, it is very difficult to obtain a fully focused image of the surface of the sample. For the optical imaging of the microscope The system requirements are very strict. In the field of digital microscopy, the most commonly used technology to solve this kind of problem is the multi-focus image sequence technology. This techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/32G06T5/00G06T17/00
CPCG06T7/32G06T17/00G06T2200/08G06T2207/10056G06T2207/10016G06T2207/20032G06T2207/20016G06T5/94G06T5/70
Inventor 郑驰萨尔瓦多·加西亚·博纳张克奇
Owner NINGBO YONGXIN OPTICS
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