Artificial cochlea electric stimulation device for nerve cell culture system
A cochlear implant and culture system technology, applied in the field of electrical stimulation, can solve the problems of cytotoxicity, reduced cell survival rate, and can not simulate physiological conditions well
Active Publication Date: 2017-12-01
SOUTHEAST UNIV
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Problems solved by technology
Common electrical stimulation devices used in cell culture systems will generate excess charges in the culture system, causing cytotoxicity and reducing the survival rate of cells; common electrical stimulation devices used in cell culture systems only emit one frequency at a time, Nerve cells are in a complex micro-electric field in the body; common electrical stimulation devices used in cell culture systems emit electrical signals directly, while human auditory neurons activate or inhibit the activity of nerve cells after being stimulated by sound, etc. Good simulation of physiological conditions
Method used
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Embodiment
[0013] The cochlear implant electrical stimulation device used in the nerve cell culture system of this embodiment includes a circuit board 1 and a stimulation dish 6; the circuit board 1 is provided with a cochlear implant 2, and the negative electrode on the circuit board 1 passes through the reference electrode 3 of the cochlear implant 2 Ion buffer connection, the positive electrode on the circuit board 1 is connected to the 22 stimulation electrodes 4 of the cochlear implant 2 through the ion buffer and covered by gelatin sponge, the negative electrode on the circuit board 1 is led out through the conductive wire 5 and is connected to the common stimulation dish 6 The conductive metal wire 8 is connected. The positive electrode on the circuit board 1 is integrated through the conductive wire 5 and then exported and connected to the platinum wire electrode 7 of the stimulation dish 6; the graphene layer 10 at the bottom of the stimulation dish 6 is exported through the inert ...
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The invention discloses an artificial cochlea electric stimulation device for a nerve cell culture system. A circuit board is provided with an artificial cochlea. A negative pole on the circuit board is connected with a reference electrode of the artificial cochlea through an ion buffer solution, and a positive pole on the circuit board and 22 stimulation electrodes of the artificial cochlea are connected through an ion buffer solution and covered with gelatin sponge. The negative pole on the circuit board is led out through conduction wires and connected with a common conductive metal wire of a stimulation vessel. A graphene layer at the bottom of the stimulation vessel is led out through an inert conductive metal wire and connected with the common conductive metal wire. A platinum wire electrode is inserted into the stimulation vessel, and a circuit loop system is formed through a conductive cell culture solution. The artificial cochlea electric stimulation device has the characteristics that the device is designed special for the neural stem cell / auditory neuron-graphene culture system, the charge accumulation effect generated by the electric stimulation device is avoided, the multi-frequency sonic-electric transformation characteristic can be applied to a nervous system, and real physiological conditions are simulated.
Description
Technical field [0001] The invention relates to an electrical stimulation technology for a nerve cell culture system, in particular to an artificial cochlear electrical stimulation device for a nerve cell culture system. Background technique [0002] Electrical stimulation technology has been relatively rare in clinical applications, but it has a significant role in cell biology research, especially in neural cell culture systems. Common electrical stimulation devices used in cell culture systems will generate excess charges in the culture system, causing cytotoxicity and reducing the survival rate of cells; common electrical stimulation devices used in cell culture systems only emit one frequency at a time, While nerve cells are in a complex micro-electric field in the body; common electrical stimulation devices used in cell culture systems directly emit electrical signals, while human auditory neurons activate or inhibit the activity of nerve cells after being stimulated by sou...
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CPCC12M35/02
Inventor 郭荣荣唐明亮柴人杰张琦罗乐
Owner SOUTHEAST UNIV