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Saccharomyces cerevisiae GA8 and application thereof

A technology of bio-control yeast and bio-control agent, applied in the application, biocide, microorganism and other directions, can solve the problems of pathogen resistance, chemical agent affecting human health, environmental pollution, etc. , the effect of good colonization ability

Inactive Publication Date: 2017-12-08
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical agents have disadvantages such as affecting human health, polluting the environment, and causing drug resistance of pathogenic bacteria.

Method used

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  • Saccharomyces cerevisiae GA8 and application thereof
  • Saccharomyces cerevisiae GA8 and application thereof
  • Saccharomyces cerevisiae GA8 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and Identification of Biocontrol Yeast GA8

[0026] (1) Test method

[0027] will be 10 5 cfu / mL Botrytis cinerea spore suspension was spread evenly on the PDA plate, a circular filter paper piece with a diameter of 6 mm was placed in the center of the petri dish, and 10 μL of yeast suspension (10 μL) was dropped on the circular filter paper piece. 8 cfu / mL), cultured statically at 27°C for 7 days, and observed the growth status of Botrytis cinerea.

[0028] The surface of the biocontrol yeast GA8 colony on PDA medium is smooth and opaque, yellowish, round, with neat edges and creamy, see figure 1 .

[0029] The DNA of the strain was extracted, the 26S rDNA sequence was amplified, and sequenced. The obtained sequence was as follows. The result of comparing the sequence with the NCBI database showed that the matching degree with Saccharomyces cerevisiae was 100%.

[0030] The strain has been preserved in the General Microbiology Center of the Chin...

Embodiment 2

[0036] The inhibitory activity of embodiment 2 biocontrol yeast GA8 to Botrytis cinerea spore germination

[0037] (1) Test method

[0038] Dilute the yeast fermentation broth with physiological saline to a concentration of 10 5 cfu / mL, 10 6 cfu / mL, 10 7 cfu / mL bacterial suspension; centrifuge the yeast fermentation broth at 6000rpm for 10min and filter to obtain a cell-free filtrate, using sterile water as a control. After the above treatment, add 100 μL of the above treatment solutions, 400 μL of PDB medium (PDA medium without agar), and 100 μL of Botrytis cinerea spore suspension into sterile centrifuge tubes, mix well and place at 25 °C Constant temperature incubator 24h, each handles three repetitions, under the microscope statistics Botrytis cinerea spore germination rate, each handles and observes 100 spores (mycelium length exceeds the radius of spore and is considered as germination).

[0039] The spore germination inhibition rate was calculated according to the f...

Embodiment 3

[0046] The colonization dynamics of embodiment 3 biocontrol yeast GA8 on grapes

[0047] (1) Test method

[0048] Colonization dynamics of biocontrol yeast GA8 on grape wounds: the grapes were sterilized by immersing them in 75% alcohol for 3 minutes, then rinsed with sterile water three times, and dried in the air. Use a sterilized inoculation needle to pierce a hole (3mm long × 3mm wide × 3mm deep) in the treated grape waist, and inoculate 10 6 cfu / mL yeast suspension. After inoculation, dry it on a sterile ultra-clean bench, place it on a plate, and place it at 4°C. The number of yeasts measured 1 hour after inoculation was taken as the initial value, and the number of yeasts was measured every 1 day for a total of 6 days. Pulp tissue with a diameter and height of 7 mm was taken from the wound with a sterilized punch, ground with 5 mL of sterile water, and the number of yeasts in the grape wound was counted by the dilution plate counting method. Each treatment was repea...

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Abstract

The invention discloses Saccharomyces cerevisiae GA8 and application thereof. A strain is classified and named as Saccharomyces cerevisiae, is named as the Saccharomyces cerevisiae GA8 and was preserved in China General Microbiological Culture Collection Center (CGMCC) in June 8th, 2017, with a preservation number of CGMCC No.14225. The application comprises the following procedures: preparing an antibiological inoculant containing the Saccharomyces cerevisiae GA8; spraying the antibiological inoculant on picked grapes. The Saccharomyces cerevisiae disclosed by the invention has strong antagonism on Botryis cinerea and can be used for preventing and controlling the Botrytis cinerea of the picked grapes. The biological prevention and control method for the Botrytis cinerea of the picked grapes, provided by the invention, has the advantages of no toxicity, no pesticide resistance, environment friendliness and the like, and has a good application prospect.

Description

technical field [0001] The invention relates to the application of Saccharomyces cerevisiae biocontrol strain GA8 in the prevention and treatment of postharvest gray mold of grapes, belongs to the technical field of prevention and control of postharvest diseases of fruits and vegetables, and is specially used for the prevention and treatment of postharvest gray mold of grapes. Background technique [0002] In 2015, my country's grape planting area was 830,000 hectares, with an annual output of 12.6 million tons (including table grapes), ranking first in the world in output and second in the world in planting area. The ripening period of grapes in my country is mainly concentrated in July-October each year, and a large number of grape fruits need to be stored and kept fresh before being introduced to the market. Therefore, the safe storage and preservation of grapes after harvest is related to the development of my country's grape industry. At present, the pathogenic bacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18A01N63/04A01P3/00C12R1/865
CPCA01N63/30C12N1/18C12N1/185C12R2001/865
Inventor 刘延琳刘宗灵杜爽任艺叶冬青秦义宋育阳
Owner NORTHWEST A & F UNIV
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