Shaking-standing liquid fermentation method for improving yield of mili fungus carotenoid
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A carotene and liquid fermentation technology, applied in fermentation and other directions, can solve the problem of low carotenoid production, achieve the effect of increasing carotenoid production, low cost, and simple operation
Inactive Publication Date: 2017-12-08
SOUTH CHINA AGRI UNIV
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[0009] The technical problem to be solved in the present invention is to overcome the technical deficiency of the low carotenoid yield in the existing liquid fermentation treatment of Myromycetes, and provide a new shaking flask-static liquid fermentation for improving the carotenoid production in Myrrhia. method
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Embodiment 1
[0031] Example 1 Optimization of culture conditions
[0032] According to the relevant literature reports, taking the total amount of carotenoids produced by Myrrhizobacteria as an index, the single factor experiment was designed to investigate the days of dark fermentation, the days of static light, the light intensity, and the amount of pouring into the plate.
[0033] (1) Dark fermentation days
[0034] Inoculate two pieces of activated Cordyceps militaris in PDA liquid medium in dark shake flasks for 4-5 days to prepare liquid seeds. According to the inoculation amount of 5%, it was inoculated into the fermentation medium (rice flour 1.2%, corn flour 0.6%, soybean flour 0.3%, MgSO 4 ·7H 2 O 0.1, KH 2 PO 4 0.1, VB 1 0.001g), 150rpm, 25℃ dark shake flask fermentation, take different fermentation days 3, 6, 9, 12d and pour it into the plate for 10d, and the amount of pouring into the plate (R=11.5cm, h=2.3cm) is 100mL, and the light intensity 3000 lux, 50 ℃ drying to ...
Embodiment 2
[0053] Example 2 Response surface analysis
[0054] On the basis of the experimental data of the above-mentioned Example 1, A dark fermentation days d (3 (-1), 6 (0), 9 (1)), B light days d (6 (-1), 9 (0) ), 12(1)) and C plate pour volume mL (60(-1), 120(0), 180(1)) three factors and three levels for response surface analysis.
[0055] The parameter design and results are shown in Table 5 below:
[0056] Table 5. Response surface test scheme and results
[0057] test number
Dark fermentation days d
Days of light
Plate pour volume mL
Carotenoid content μg / g
1
-1
-1
0
608.3
2
1
-1
0
380.8
3
-1
1
0
978.3
4
1
1
0
690.8
5
-1
0
-1
950
6
1
0
-1
580
7
-1
0
1
722.5
8
1
0
1
473.3
9
0
-1
-1
485
10
0
1
-1
1003.3
11
0
-1
1
357.5
12
0
1
1
561.7
1...
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Abstract
The invention discloses a shaking-standing liquid fermentation method for improving the yield of mili fungus carotenoid. The content of the mili fungus carotenoid is creatively used as the index, after researching and summarizing, the optimum culture conditions are obtained as follows: shaking fermentation is conducted for 4 days in the dark, and illumination standing culture is conducted for 10 days; and under the conditions that a flat plate is adopted in illumination culture, wherein the flat plate has the specifications that R is equal to 11.5 cm and h is equal to 2.3 cm, 100 mL of culture media are poured into the flat plate, and the illumination intensity of illumination culture is 500 lux, the yield of the mili fungus carotenoid is maximum, and the stable technical effect is kept. The shaking-standing liquid fermentation method is easy to operate, low in cost and capable of saving energy; and through a method of combining a single factor and an orthogonal test, it is verified that the shaking-standing liquid fermentation method can improve the yield of the carotenoid in mili fungi significantly and has wide market application prospects.
Description
technical field [0001] The invention relates to the technical field of artificial cultivation of Myriads, more particularly, to a liquid fermentation method for improving the yield of Mydross carotenoids. Background technique [0002] In traditional Chinese medicine, some Cordyceps fungi, such as Cordyceps sinensis, Myriads, and Cordyceps grandis, can often be directly used as medicine in their complexes with their hosts. Cordyceps sinensis is a traditional precious Chinese medicinal material in my country. So far, people still have not realized the large-scale artificial cultivation of Cordyceps sinensis. Especially in recent years, with the rising market price, Cordyceps sinensis has been over-exploited, and its wild resources have been continuously reduced, and it has been included in the "National Second-Class Protected Species". [0003] In terms of medicinal value, Miriamia is very similar to Cordyceps sinensis and contains biologically active substances such as cordy...
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