Culture medium for detecting microorganisms in human blood and preparing method for culture medium

Abstract

The invention relates to the technical field of biological detection, in particular to a culture medium for detecting microorganisms in human blood and a preparing method for the culture medium. The culture medium for detecting microorganisms in human blood comprises a basic culture medium and additives; the additives comprise the following substances of, by mass, 5-15 parts of peptone, 2.5-7.5 parts of sodium chloride, 1-3 parts of glucose, 0.5-4 parts of lactose, 1.5-3.5 parts of phosphorus source, 0.01-0.08 part of bile salt pig, 0.5-6 parts of mannitol, 1-3 parts of sodium pyruvate, 1-6 parts of amino acid, 1-8 parts of beta-nuclear glycosidase substrate, 1-3 parts of mango extract, 0.02-0.58 part of metal salt, 0.01-0.05 part of surfactant, 7.2-7.5 of pH value, and 1000 parts of distilled water. According to the culture medium for detecting microorganisms in human blood, the true content of different microorganisms in blood can be directly inferred by colony counting and using solid culture medium, whether the blood is in a carrier state or has reached the pathogenic concentration can be determined from the quantitative point of view, so that veracity of detecting results is further improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a culture medium for detecting microorganisms in human blood and a preparation method thereof. Background technique [0002] Normal human blood is free of bacteria. When the human body is infected by bacteria, according to the degree of bacterial infection, the diseases caused by blood bacterial infection can be simply divided into bacteremia, sepsis, sepsis and so on. Bacterial infection of the human body generally causes inflammation in a certain part first, such as boils, carbuncles, pneumonia, encephalitis, etc., in the case of reduced resistance. In medicine, the disease caused by bacteria entering the blood circulation from the local inflammation site is called bacteremia. At this time, a small amount of bacteria will often invade the blood. If the person's general condition is good, this small amount of bacteria will be eliminated by various antibodies, phag...

AI Technical Summary

Problems solved by technology

However, in actual clinical tests, some specific pathogenic bacteria often fail to be detected.

Especially in recent years, the incidence of blood infection is on the rise. Many hospitals with better equipment basically use automatic blood bacterial culture equipment for blood bacterial culture. Generally, the positive rate of blood culture is not high enough, and the culture period is long, which is prone to Contamination leads to problems such as erroneous results, which have a huge impact on the selection of drugs in clinical treatment, timely and effective infection control, and the final patient treatment effect