Method for distinguishing Dai-qu stock pseudosciaena crocea from Fujian-eastern Guangdong stock pseudosciaena crocea, based on fatty acid composition difference

Abstract

The invention discloses a method for distinguishing Dai-qu stock pseudosciaena crocea from Fujian-eastern Guangdong stock pseudosciaena crocea, based on the fatty acid composition difference. The method comprises the steps of collecting muscle of pseudosciaena crocea, performing saponification and methyl esterification after total lipids are extracted, performing qualification and quantification on fatty acid in the muscle after GC-MS analysis, and judging collected pseudosciaena crocea as Dai-qu stock pseudosciaena crocea when the fatty acid [C18:1 / (C20:1+C22:1)] in the muscle is smaller than 5.0; judging collected pseudosciaena crocea as Fujian-eastern Guangdong stock pseudosciaena crocea when the fatty acid [C18:1 / (C20:1+C22:1)] in the muscle is greater than 5.0. The method has the advantages that the distinguish degree is increased through a quantitative ratio of a fatty acid marker according to the fact that Dai-qu stock pseudosciaena crocea has a fatty acid marker which is obviously different from that of Fujian-eastern Guangdong stock pseudosciaena crocea, and a technical support is provided for the genetic resource conservation, selective breeding and marketing and sales of Dai-qu stock pseudosciaena crocea.

Description

technical field [0001] The invention relates to a method for identifying the species of the large yellow croaker, in particular to a method for identifying the large yellow croaker of the Daiqu nationality and the Fujian-Yuedong nationality based on the difference in fatty acid composition. Background technique [0002] Large yellow croaker (Larimichthys crocea), belonging to Perciformes (Perciformes), Sciaenidae (Sciaenidae), and Larimichthys (Larimichthys), is a group of inshore fish that can migrate in groups, traditionally divided into 3 geographical groups : Daiqu, Min-Yuedong and Naozhou. Wild Daiqu large yellow croaker resources are on the verge of depletion due to overfishing. Most of the large yellow croakers bred in Ningbo are Fujian-Yuedong large yellow croakers. The quality and flavor of the Fujian-Yuedong large yellow croaker is not as good as that of the local Daiqu large yellow croaker. The latter also has advantages in terms of growth speed and cold resistan...

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Problems solved by technology

Wang Dexiang et al. (Wang Dexiang, Wang Jun, Su Yongquan. Identification primers and identification methods for different populations of large yellow croaker [P]. Chinese patent: 200610135259, 2006.11.28.) and Ding Shihua et al. (Ding Shihua, Huang Liying, Zhang Haiqi, Xu Xiaolin. Large yellow croaker (Pseudosciaena Crocea) Genetic difference analysis between Dai Quyang breeding population and Guanjingyang breeding population [J]. Ocean and Limnology, 2006, 37(1): 41-46.) Using single primer RAPD-PCR amplification pattern to distinguish Dai Qu family large yellow croaker and Fujian-Yuedong large yellow croaker, however, RAPD-PCR technology has high requirements on the quality of DNA templates, amplification conditions and systems, and its inherent defects are poor reproducibility and difficult overcome

Aiming at the difference loci in the D-loop and ND4 regions of Daiqu large yellow croaker and Fujian-Guangdong large yellow croaker, combined analysis of the two regions after OTU typing was used to identify Daiqu large yellow croaker (Shen Xiquan, He Mengmeng, Yan Xiaojun, et al. Amplification of the complete mitochondrial genome sequence of Daiqu large yellow croaker (Pseudosciaena crocea) and the development of specific identification primers [J]. Ocean and Limnology, 2013, 44(3): 755-762.), their experiment The data is highly repeatable and the identification accuracy is high, but the above method still has a small probability of misjudgment

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