Detection method of total content of fructo-oligosaccharide in formula milk/milk powder
A technology of fructooligosaccharides and detection methods, which is applied in the detection field of the total amount of fructooligosaccharides, can solve the problems of small application range, high price, and limited popularization and application, and achieves the advantages of strong versatility, low equipment cost and high popularization Effect
Inactive Publication Date: 2017-12-08
NINGBO INST FOR FOOD CONTROL
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Only the amount of three kinds of fructo-oligosaccharides represents the total amount of fructo-oligosaccharides, which is obviously inappropriate for the detection of products added with fructo-oligosaccharides derived from chicory or inulin
[0004] The national standard GB5009.255-2016 implemented on March 1, 2017 is a method for the detection of fructans in food, which can effectively detect the total amount of fructo-oligosaccharides. However, this method requires ion chromatography with ternary and For the detection of the above gradient pump and pulse amperometric detector, the cost of the equipment used is more than one million yuan, and the gradient pump and pulse amperometric detector of ternary and above are highly specific and have a small scope of application, so this standard method is limited. Promoted application of
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[0050] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0051] The samples to be tested were 4 batches of infant formula milk powder submitted by a certain company, among which the samples labeled 1 and 2 were marked as containing sucrose, and the samples 3 and 4 were marked as sucrose-free.
[0052] The detection method of fructo-oligosaccharide total amount, comprises the following steps:
[0053] 1) Weigh the fructose standard substance and dilute it step by step with distilled water, and dilute it into standard solutions of 6 different concentrations (50mg / L, 80mg / L, 100mg / L, 150mg / L, 200mg / L, 300mg / L concentration), The standard solutions of different concentrations are detected in high performance liquid chromatography-evaporative light detector (Agilent 1260Infinity), and the corresponding chromatographic peak area values are measured to obtain standard spectrograms, wherein the chromatogr...
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Abstract
The invention discloses a detection method of total content of fructo-oligosaccharide in formula milk/milk powder. The detection method realizes enzymolysis treatment of the fructo-oligosaccharide by utilizing an enzymolysis method, and moreover realizes detection of the total content of the fructo-oligosaccharide in the formula milk/milk powder by adopting a universal high performance liquid chromatography-evaporative photodetector by selecting a specific chromatographic condition. The detection method has the advantages that the trouble that a special ampere detector for ion chromatography impulse is required to be used in the existing national standard method GB5009.88-2016 is overcome; the equipment cost is low; the universality is high; and the generalization performance is high. Compared with a detection result of the special ampere detector for the ion chromatography impulse used by the method GB5009.88-2016, an error is less than 5%.
Description
technical field [0001] The invention relates to a method for detecting the total amount of fructo-oligosaccharides, in particular to a method for detecting the total amount of fructo-oligosaccharides in formula milk / milk powder. Background technique [0002] Fructose-oligosaccharide is a natural active substance, which has the functions of regulating intestinal flora, proliferating bifidobacteria, promoting calcium absorption, regulating immunity, and anti-caries. It is an important part of soluble dietary fiber and is widely used due to its good performance Added to formula food and supplementary food. For example, as a nutritional fortifier, the GB14880-2012 standard clearly stipulates that the maximum limit of its use in infant formula food should not exceed 64.5g / kg. [0003] The current literature research shows that the detection methods of fructooligosaccharides are mainly liquid chromatography and ion chromatography. Most of them use kestose, kestose and kestose pe...
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/067
Inventor 张书芬李湘江邢家溧毕晓丽沈坚应璐周子焱张爱芝张少华章银珠
Owner NINGBO INST FOR FOOD CONTROL
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