Detection method of total content of fructo-oligosaccharide in formula milk/milk powder

A technology of fructooligosaccharides and detection methods, which is applied in the detection field of the total amount of fructooligosaccharides, can solve the problems of small application range, high price, and limited popularization and application, and achieves the advantages of strong versatility, low equipment cost and high popularization Effect
CN107449840AInactive Publication Date: 2017-12-08NINGBO INST FOR FOOD CONTROL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGBO INST FOR FOOD CONTROL
Publication Date
2017-12-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a detection method of total content of fructo-oligosaccharide in formula milk / milk powder. The detection method realizes enzymolysis treatment of the fructo-oligosaccharide by utilizing an enzymolysis method, and moreover realizes detection of the total content of the fructo-oligosaccharide in the formula milk / milk powder by adopting a universal high performance liquid chromatography-evaporative photodetector by selecting a specific chromatographic condition. The detection method has the advantages that the trouble that a special ampere detector for ion chromatography impulse is required to be used in the existing national standard method GB5009.88-2016 is overcome; the equipment cost is low; the universality is high; and the generalization performance is high. Compared with a detection result of the special ampere detector for the ion chromatography impulse used by the method GB5009.88-2016, an error is less than 5%.
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Description

technical field

[0001] The invention relates to a method for detecting the total amount of fructo-oligosaccharides, in particular to a method for detecting the total amount of fructo-oligosaccharides in formula milk / milk powder. Background technique

[0002] Fructose-oligosaccharide is a natural active substance, which has the functions of regulating intestinal flora, proliferating bifidobacteria, promoting calcium absorption, regulating immunity, and anti-caries. It is an important part of soluble dietary fiber and is widely used due to its good performance Added to formula food and supplementary food. For example, as a nutritional fortifier, the GB14880-2012 standard clearly stipulates that the maximum limit of its use in infant formula food should not exceed 64.5g / kg.

[0003] The current literature research shows that the detection methods of fructooligosaccharides are mainly liquid chromatography and ion chromatography. Most of them use kestose, kestose and kestose pe...

Claims

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