Characteristic spectrums of lung cancer and pulmonary nodule proteins as well as construction methods and application of characteristic spectrums

Abstract

The invention discloses characteristic spectrums of lung cancer and pulmonary nodule proteis as well as construction methods and application of the characteristic spectrums. The methods comprise the following steps of extracting total protein from the plasma of a lung cancer/pulmonary nodule patient group and a healthy control group respectively, carrying out enzymolysis by trypsase, carrying out iTRAQ (isobaric Tags for Relative and Absolute Quantification) labeling, carrying out two-dimensional liquid chromatographic and tandem mass spectrometric detection, and carrying out relative quantitative analysis, so that the characteristic spectrums of the proteins related to the lung cancer/pulmonary nodule are obtained, wherein in the characteristic spectrum of the protein related to the lung cancer, the protein with expression differences in the lung cancer patient group and the healthy control group is up-regulated protein (SAA1 (Serum Amyloid A 1), SERPINA1 (Alpha-1-Antitrypsin), CRP (C-Reactive Protein), IGHG4 (Human Immunoglobulin G 4) and a CFH (Complement Factor H)); in the in the characteristic spectrum of the protein related to the pulmonary nodule, the protein with expression differences in the pulmonary nodule patient group and the healthy control group is up-regulated protein (the SERPINA1 and the CRP) and down-regulated protein (the SAA1, the IGHG4 and the CFH). The characteristic spectrums are used for facilitating the research of the biological natures of the lung cancer and the pulmonary nodule, and have important significance for the normative typing of the lung cancer and the pulmonary nodule.

Description

technical field [0001] The invention belongs to the field of medicine and proteomics, and relates to a characteristic spectrum of lung cancer and pulmonary nodule protein and its construction method and application. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. Pulmonary nodule is a pulmonary granulomatous disease with unknown etiology, which has attracted widespread attention in China recently. [0003] Currently, lung cancer diagnosis methods include X-ray examination, bronchoscopy, cytology examination, thoracotomy, ECT examination, me...

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Problems solved by technology

In an acute time-limited reaction, stimulated by IL-1, IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, which can increase to 100-1000 times the initial concentration, but the half-life is extremely short. Short, only about 50 minutes

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