Method for producing spirulina biomass by using natural seawater and aginomoto for fermenting mother liquor

A technology of spirulina and biomass, applied in the direction of adding compounds to stimulate growth, bacteria, etc., can solve problems such as spirulina biomass that has not been found, achieve good application value, increase unit protein content and yield, and high efficiency Effect

Inactive Publication Date: 2017-12-12
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has been no report of using natural seawater to add monosodium glutamate fermented yeast liquid to produce spirulina biomass

Method used

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  • Method for producing spirulina biomass by using natural seawater and aginomoto for fermenting mother liquor
  • Method for producing spirulina biomass by using natural seawater and aginomoto for fermenting mother liquor

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Embodiment

[0055] 1. Algae culture process:

[0056] (1) Inoculate Spirulina algae species in Zarrouk medium and carry out activation culture for 3 days to obtain activated algae cells. The components of Zarrouk medium are as follows (g.L -1 ): NaNO 3 ,2.5; NaCl, 0.5; MgSO 4 ·7H 2 O, 0.15; CaCl 2 2H 2 O, 0.04; calcium superphosphate (Ca(H 2 PO 4 ) 2 ·CaSO 4 ·H 2 O), 1.25; KCl, 0.898; NaHCO 3 ,16.8;

[0057] (2) Inoculate the activated cells prepared in step (1) in Zarrouk medium and expand the culture to the logarithmic growth phase; before inoculation, sieve the enriched algae cells and wash them with deionized water to obtain the algae mud to be inoculated with spirulina;

[0058] (3) Add different proportions of monosodium glutamate fermented yeast liquid (50:1 to 800:1, V seawater: V monosodium glutamate fermented yeast liquid) in seawater to prepare microalgae culture liquid; resuspend appropriate amount of algae mud with culture liquid, initial OD 680 0.2, in an artifi...

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Abstract

The invention firstly provides a new method for culturing spirulina without freshwater consumption by adding aginomoto for fermenting mother liquor under the condition of natural seawater. According to the method, the growth inhibition effect of the natural seawater to the spirulina is effectively relieved, the yield of the spirulina biomass is obviously increased, the unit protein content and the yield of the spirulina are obviously increased and a high-quality protein source is supplied to people. According to the method provided by the invention, the operation is simple, the time consumption is short, the efficiency is high and the cost is low, and the method easy to the realize, has an excellent application value and supplies an effective technical scheme for the large-scale development and utilization of the spirulina.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for producing spirulina biomass by using natural seawater plus monosodium glutamate fermented yeast liquid. Background technique [0002] Spirulina biomass is rich in nutrition, rich in protein, unsaturated fatty acids and multivitamins, and the protein is of high quality, including more than 20 kinds of amino acids needed by human body and animals. It is currently known as a food resource with high protein content and rich nutrition. It has extremely high nutritional value and the function of improving immunity. It is listed by the Food and Agriculture Organization of the United Nations (FAO) as an important protein source for humans in the 21st century. In addition, it is reported that Spirulina has biologically active substances that inhibit tumors, fight cancer, enhance immunity, and resist viral infections. Its growth factors also have the effect of enhancing...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20
CPCC12N1/20C12N1/38
Inventor 裴海燕蒋丽群李亦真聂昌亮
Owner SHANDONG UNIV
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