Multistage protein separation and collection equipment with improved structure

Abstract

The invention discloses multistage protein separation and collection equipment with an improved structure. The multistage protein separation and collection equipment comprises a shell, wherein a separating plate is arranged inside the shell, the shell comprises an upper chamber and a lower chamber, a filtration unit, a chromatographic column and a first pump body are arranged in the lower chamber of the shell, one end of the filtration unit is connected with a feeding pipe, the feeding pipe penetrates through the side wall of the shell and extends to the exterior of the shell, a first control valve and a flow meter are arranged on the feeding pipe, the first control valve and the flow meter are arranged outside the shell, and a filtration membrane is arranged inside the filtration unit. The multistage protein separation and collection equipment disclosed by the invention has the advantages that multistage protein separation requirement is realized by virtue of combination of multiple separation equipment, separation and purification of mixed complex proteins are solved, continuous efficient separation is formed, and separation efficiency is improved; an S-shaped circulating pipeline and a heater are arranged on the side wall of the shell, so that protein separation solubility is improved; and multiple separation devices are arranged in one shell, so that structure is compact, and occupying space is small.

Description

technical field [0001] The invention relates to the technical field of separation and purification of pharmaceutical proteins, in particular to a multi-stage protein separation and collection device with improved structure. Background technique [0002] Protein separation generally uses a chromatographic column alone, which is generally a method of separation based on the different distribution coefficients of the components in the sample mixture in the stationary phase and the mobile phase. The existing separation methods usually use packed colloidal chromatography columns. Protein separation is carried out through hydrophilic-hydrophobic relationship, etc. However, when the protein components are complex and fine separation is required, it is difficult to achieve effective and high-purity separation. It is easy to mix again, so we propose a multi-stage protein separation and collection device with improved structure to solve the above problems. Contents of the invention ...

AI Technical Summary

Problems solved by technology

[0002] Protein separation generally uses a chromatographic column alone, which is generally a method of separation based on the different distribution coefficients of the components in the sample mixture in the stationary phase and the mobile phase. The existing separation methods usually use packed colloidal chromatography columns. Protein separation is carried out through hydrophilic-hydrophobic relationship, etc. However, when the protein components are complex and fine separation is required, it is difficult to achieve effective and high-purity separation. It is easy to mix again, so we propose a multi-stage protein separation and collection device with an improved structure to solve the above problems

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