A pandoraea sp. strain and an application method thereof

A technology of pandora bacteria and strains, applied in the field of environmental pollution control, can solve problems such as environmental pollution, and achieve the effect of good degradability

Active Publication Date: 2017-12-15
UNIV OF SCI & TECH LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Pandora bacterium has a good degradation effect on n-hexadecane, monocyclic benzene series and gasoline petroleum hydrocarbons, and can quickly and completely deg

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Under aseptic conditions, add 0.5mL gasoline to 100mL inorganic salt medium, and then inoculate the microorganism Pandora strain JWJ-06. The initial inoculation of the strain is 1.25×10 7 Then put the culture medium on a shaker at a rotation speed of 120rad / min, maintain the pH of the culture medium at 6-8, temperature 30±1°C, and incubate for 120h, the gasoline degradation rate was measured to be 53.2%.

Embodiment 2

[0061] Under aseptic conditions, add 0.35mL gasoline to 100mL inorganic salt medium, and then inoculate the microorganism Pandora strain JWJ-06. The initial inoculation amount of the strain is 2×10 7 Then put the culture medium on a shaker at a rotation speed of 100rad / min, maintain the pH of the culture medium at 6-8, temperature 29±1°C, and culture time 96h, and the measured gasoline degradation rate is 62.0%.

Embodiment 3

[0063] Under aseptic conditions, add 1.2mL gasoline to 100mL inorganic salt medium, and then inoculate the microorganism Pandora strain JWJ-06. The initial inoculation amount of the strain is 5×10 6 Then put the culture medium on a shaker at a rotation speed of 120 rad / min, and maintain the pH of the culture medium at 6-8, a temperature of 29±1°C, and a culture time of 108 hours. The gasoline degradation rate was measured to be 46.5%.

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PUM

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Abstract

The objective of the invention is to develop a microbiological method for treating gasoline type petroleum hydrocarbons, and the invention provides a pandoraea sp. strain and an application method thereof. The strain is pandoraea sp. JWJ-06, and is called pandoraea sp. JWJ-06 for short, and the accession number of the pandoraea sp. is CGMCC No.13558. The application method includes degrading organic compounds or waste water containing organic compounds by adopting the pandoraea sp.. The organic compounds are gasoline type petroleum hydrocarbons, n-hexadecane, toluene, or a mixture of two or three of the gasoline type petroleum hydrocarbons, the n-hexadecane and the toluene. The pandoraea sp. has good degradation effects on the n-hexadecane, single-ring benzene compounds and the gasoline type petroleum hydrocarbons, can rapidly and relatively-completely degrade the n-hexadecane, the single-ring benzene compounds and the gasoline type petroleum hydrocarbons, and provides a novel route for solving a problem that gasoline or other petroleum hydrocarbons pollutes the environment.

Description

Technical field [0001] The invention belongs to the field of environmental pollution control, and particularly relates to a Pandora bacterium that degrades petroleum hydrocarbons and a method of use thereof. Background technique [0002] With the continuous development of industry, petroleum is used as one of the main energy sources and one of the main raw materials in various industries. However, in the course of oil exploitation, transportation, processing, etc., due to problems such as leakage and wrong discharge, soil, rivers, groundwater, oceans, etc. are contaminated by oil, causing pollution to the ecological environment. [0003] The remediation techniques for petroleum hydrocarbon pollution mainly include physical, chemical and microbiological methods. Compared with physical remediation and chemical remediation, microbial remediation technology is a more mature technology, and it has the advantages of safety, high efficiency, low cost, simple operation and no secondary po...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C02F3/34A62D3/02C12R1/01C02F101/32A62D101/20
CPCA62D3/02A62D2101/20C02F3/343C02F2101/32C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 金文杰郝瑞霞雷春燕刘莹邢齐琪王旗聂振皓
Owner UNIV OF SCI & TECH LIAONING
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