A method for rapid extraction of plant genome dna
A plant genome and extraction method technology, applied in the field of rapid extraction of plant genome DNA, can solve the problems of cumbersome operation, long time-consuming, unstable quality of extracted DNA, etc., achieve good DNA integrity, improve purity, and shorten the extraction time.
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[0031] The present embodiment compares with the method for quickly extracting plant genomic DNA of the present invention and the traditional method for extracting genomic DNA of Radix radix:
[0032] Plant genomic DNA rapid extraction method of the present invention, comprises the following steps:
[0033] (1) Take 100 mg of fresh corn leaf sample and add liquid nitrogen to grind it into powder, and collect it into a 1.5 ml sterile centrifuge tube.
[0034] (2) Add 600ul of BufferA to the centrifuge tube, quickly invert and mix well, then place the centrifuge tube in a 70°C water bath for 10-30 minutes, during which time the centrifuge tube is inverted several times to mix the plant tissue sample with BufferA. Buffer A was preheated at 70°C before adding to the centrifuge tube.
[0035] Further, the Buffer A contains Tris-HCl, Na 2 EDTA, NaCl, SDS and PVP40; and the concentration of Tris-HCl in Buffer A per 1000ml is 50-100mM, Na 2 The concentration of EDTA is 10-50mM, the ...
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