Method for constructing Parkinson's disease model mouse by means of unilateral two-point injection method

Abstract

The invention discloses a method for constructing a Parkinson's disease model mouse by unilateral two-point injection. The method comprises the following steps: Step 1. After the rat is anesthetized by intraperitoneal injection, the hair on the top of the head is cut off, and the scalp and subcutaneous tissue are cut off. Tissue and periosteum; step 2, use a dental drill or a syringe needle to carefully drill through the skull at the location so as not to damage the dura mater, connect the micro-inhalation drug syringe to the micro-propeller with the determined coordinates, and inject the configured drug into the SNc and VTA 6‑OHDA 5 μl, vertically into the cranium; step 3, after suturing, 50,000 units of penicillin were injected intraperitoneally for three consecutive days to prevent infection, and animal models were established; step 4, after 10 days, intraperitoneal injection of APO induced rats to rotate to the left . The method of the present invention uses a needle to puncture the rat skull during the model making process, can effectively save model making time, is convenient and fast, has simple requirements on instruments and can be more sterile.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for constructing Parkinson's disease model mice by using a unilateral two-point injection method. Background technique [0002] Parkinson's disease (PD) is a degenerative disease of the nervous system, and its typical pathological features are loss of dopamine DA neurons in the substantia nigra of the midbrain and a decrease in the number of DA in the nigrostriatal pathway. There are many ways to establish Parkinson's animal models, the most commonly used ones are rat models prepared by 6-OHDA (6-hydroxydopamine) and 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, MPTP) made mouse or primate model. Rats are usually used to make animal models. 6-OHDA-induced PD animal models have great similarities with human PD in terms of neurobiochemistry, pathology and behavior, and have been widely used in PD research. However, the extent and ...

AI Technical Summary

Problems solved by technology

The SNc area is small, and the success rate of general modeling is low

As another important target, the VTA is closely related to body movement and is an important area related to PD pathology. If it is damaged alone, the modeling rate will also be low.