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Registration method and device for microscopic images of biological tissue serial slices

A technology of microscopic images and biological tissues, which is applied in the fields of image processing and neuroscience, can solve problems such as inability to find, and achieve the effects of avoiding large registration errors, reducing calculations, and avoiding mismatching

Active Publication Date: 2020-05-19
INST OF AUTOMATION CHINESE ACAD OF SCI
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Problems solved by technology

[0006] In order to solve the above-mentioned problems in the prior art, that is, to solve the technical problem that it is impossible to find true and reliable corresponding point information between adjacent slice microscopic images, one aspect of the present invention proposes a biological tissue serial slice microscopic Image registration methods, including:

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  • Registration method and device for microscopic images of biological tissue serial slices
  • Registration method and device for microscopic images of biological tissue serial slices
  • Registration method and device for microscopic images of biological tissue serial slices

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[0047] Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principle of the present invention, and are not intended to limit the protection scope of the present invention.

[0048] The image registration method for microscopic images of serial slices of biological tissues in the embodiment of the present invention will be described below with reference to the accompanying drawings.

[0049] like figure 1 As shown, the method for registering microscopic images of serial slices of biological tissues in this embodiment includes steps S100, S200 and S300.

[0050] Step S100, acquiring corresponding points of the microscopic images of adjacent slices in the microscopic images of the serial slices of the biological tissue. Specifically, in this embodiment, the SIFT flow algorithm can be used to obtain the corr...

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Abstract

The invention relates to the field of image processing and neurosciences, in particular to a biological tissue sequence slice microscopic image registration method and apparatus, and aims to solve theproblem that real and reliable corresponding point information cannot be found between adjacent biological tissue slice microscopic images. For the purpose, the invention provides the biological tissue sequence slice microscopic image registration method. The method comprises the steps of obtaining corresponding points of adjacent microscopic images in biological tissue sequence slice microscopicimages; adjusting the positions of the obtained corresponding points; and according to the adjusted positions of the corresponding points, performing image deformation on the biological tissue sequence slice microscopic images. Through the method and the apparatus, the real and reliable corresponding point information between the adjacent slice microscopic images can be found, so that the precision of biological tissue sequence slice microscopic image registration is ensured.

Description

technical field [0001] The invention relates to the fields of image processing and neuroscience, in particular to a method and device for registering microscopic images of biological tissue serial sections. Background technique [0002] At present, the three-dimensional reconstruction methods of biological tissue serial sections microscopic images based on electron microscope imaging technology mainly include serial section imaging methods and serial section imaging methods. Among them, the steps of the sequential cross-sectional imaging method mainly include: fixing the biological tissue sample in the sample chamber of the electron microscope, removing the surface of the sample with a thickness of ten nanometers by diamond knife cutting or ion beam bombardment after imaging it, and then processing it again. The samples that have been passed are imaged, and the above process is repeated until images of all samples are obtained. The image sequences obtained by the above imag...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/00G06T7/33
Inventor 陈曦韩华谢启伟沈丽君
Owner INST OF AUTOMATION CHINESE ACAD OF SCI
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