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In-vitro rapid propagation method for North American magnolia

An in vitro and magnolia technology, applied in the field of plant breeding, can solve the problems of low survival rate and maintenance of unfavorable traits, etc., and achieve the effect of fast growth

Active Publication Date: 2018-01-09
WEIFANG VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, domestic relevant units such as Shenyang Academy of Garden Sciences, Xi'an Botanical Garden, Institute of Atomic Energy of Anhui Academy of Agricultural Sciences, Lushan Botanical Garden of Chinese Academy of Sciences, Jiangsu Provincial Institute of Forestry Sciences, etc., have carried out introduction and production development of magnolia plants, but many Using cuttings and grafting, the survival rate is not high, and it is not conducive to the maintenance of traits. The tissue culture and rapid propagation technology of North American magnolia is almost blank in China.

Method used

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  • In-vitro rapid propagation method for North American magnolia
  • In-vitro rapid propagation method for North American magnolia
  • In-vitro rapid propagation method for North American magnolia

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 A kind of in vitro rapid propagation method of Magnolia magnolia

[0030] 1. Material preparation

[0031] The experiment was carried out in the Tissue Culture Center of Weifang Vocational College from 2010 to 2012, and the experimental material was 'Sticata' magnolia ( Magnolia 'Stricta' ).

[0032] 2. Selection of explants

[0033] Select the 'Sticata' Magnolia individual plant with vigorous growth, no damage by diseases and insect pests, and good performance in the outdoor; cut the explants with scissors, the temperature is 15 ℃ when cutting the explants, and the humidity is 46%;

[0034] The cut explants are: stem section with axillary buds, requirements: the stem section is semi-lignified, with 1 full axillary bud, and the length of the stem section is 2.4cm.

[0035] 3. Disinfection of explants

[0036] Place the explants in a triangular flask, add detergent and water, shake continuously, and wash for 14 minutes; then rinse with clean water for 3 ...

Embodiment 2

[0055] The influence of embodiment 2 different parts explants and sterilization time on survival rate

[0056] Adopt the method of embodiment 1 to breed North American 'Sticata' Magnolia, only change the explant part clipped in step 2 and the mercury chloride disinfection time in step 3, carry out embodiment 2-5; Specific changes are shown in Table 1 ;

[0057] Table 1 Clipped explants and mercuric chloride disinfection time

[0058]

[0059] See Table 2 for the explant survival rate cultivated by embodiment 1-5;

[0060] Table 2 The explant survival rate of embodiment 1-5 propagation cultivation

[0061]

[0062] As can be seen from Table 2, the explant disinfection pollution rate of embodiment 3 breeding is the lowest, and the survival rate is the highest, which is a preferred embodiment; that is, the explant position is preferably a stem section with axillary buds, and the length of the stem section is preferably 2.8cm. The number is preferably 2, and the mercury c...

Embodiment 6

[0063] Example 6 Induction test of different mediums on clustered buds of Magnolia magnolia

[0064] Adopt the method of embodiment 1 to carry out tissue culture, only change the differentiation medium in step 6 differentiation culture, carry out experiment, totally 10 experimental groups, the specific differentiation medium of each experimental group is as follows:

[0065] Experimental group 1: MS medium + 6-BA 0.4 mg / L + IBA 0.08 mg / L + sucrose 25 g / L + agar 9 g / L;

[0066] Experimental group 2: MS medium + 6-BA 0.3 mg / L + IBA 0.06 mg / L + sucrose 28 g / L + agar 8g / L;

[0067] Experimental group 3: MS medium + 6-BA 0.2 mg / L + IBA 0.04 mg / L + sucrose 25 g / L + agar 9 g / L;

[0068] Experimental group 4: MS medium + 6-BA 0.1 mg / L + IBA 0.02 mg / L + sucrose 26 g / L + agar 9 g / L;

[0069] Experimental group 5: MS medium + 6-BA 0.05 mg / L + IBA 0.08 mg / L + sucrose 25 g / L + agar 9 g / L;

[0070] Experimental group 6: MS medium + 6-BA 0.3 mg / L + NAA 0.06 mg / L + sucrose 25 g / L + agar 9 ...

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Abstract

The invention provides an in-vitro rapid propagation method for North American magnolia. The in-vitro rapid propagation method comprises steps of explant selection, explant disinfection, preparation of an initiation culture medium, initiation culture, differentiation culture, cultivation of tissue culture seedlings and seedling hardening. For explant selection, a single magnolia plant is selectedoutdoors, and an explant is cut off at the temperature of 15 DEG C and the humidity of 46%; the cut-off explant is a stem segment with axillary buds, the stem segment is semi-lignified and has two full axillary buds, and the stem segment is 2.8 cm long. For initiation culture, the culture conditions are controlled as follows: the temperature is controlled at 23 DEG C plus or minus 0.5 DEG C, illumination time is 12 h / d, illumination intensity is 2500 Lx, and humidity is 50%. The survival rate of North American magnolia cultivated with the in-vitro rapid propagation method is as high as 99.5%.

Description

technical field [0001] The invention relates to a breeding method of North American magnolia, in particular to a method for in vitro rapid propagation of North American magnolia, belonging to the technical field of plant breeding. Background technique [0002] Magnolia is a deciduous tree with a height of up to 15 meters. The flowers are white, large and fragrant, the first leaves open, and the flowering period is about 10 days. Famous flowers and trees in China, important flower-viewing trees in early spring in the north. The most common ones in the north are Erqiao Magnolia, etc. The petals are lavender outside and white inside. North American Magnolia is more colorful than Chinese Magnolia, with a large number of flowers and high ornamental value. [0003] At present, domestic relevant units such as Shenyang Academy of Garden Sciences, Xi'an Botanical Garden, Institute of Atomic Energy of Anhui Academy of Agricultural Sciences, Lushan Botanical Garden of Chinese Academ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 丁世民吴祥春李寿冰郭继民杨兴芳赵从凯孙曰波郝炎辉庄德祥
Owner WEIFANG VOCATIONAL COLLEGE
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