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A preparation method of neoantigen-presenting cells for cell therapy

A cell therapy and cell technology, applied in the field of cell engineering, can solve the problems of difficulty in obtaining and limiting effects, and achieve the effect of slight rejection, high safety, and simple operation

Active Publication Date: 2018-07-31
BEIJING DCTY BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the past and current tumor-specific immunotherapy, it is hoped to obtain specific immune cells targeting tumor cells to kill tumor cells in order to obtain a better therapeutic effect. For example, DC vaccines use DC cells to carry specific antigens and reinfuse them However, the difficulty of obtaining DC cells and the tumor immune microenvironment in patients greatly limit the effect of DC vaccines

Method used

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  • A preparation method of neoantigen-presenting cells for cell therapy
  • A preparation method of neoantigen-presenting cells for cell therapy
  • A preparation method of neoantigen-presenting cells for cell therapy

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Embodiment

[0026] Such as figure 1 As shown, this embodiment provides a method for preparing neoantigen-presenting cells for cell therapy, comprising the following steps:

[0027] 1) Draw about 10mL of human peripheral blood;

[0028] 2) Separate the buffy coat layer using human peripheral blood mononuclear cell separation medium to obtain peripheral blood mononuclear cells (PBMC);

[0029] 3) Wash the cells once with PBS;

[0030] 4) Prepare RPMI-1640 medium and RPMI-27γ medium containing 600U / mL IL-2, 800U / mL IL-4, 40ng / mL IL-7 and 100pg / mL IFN-γ;

[0031] 5) Use 1 mL of prepared RPMI-27γ medium and incubate PBMC at 37°C for 2 hours;

[0032] 6) Use RPMI-1640 to prepare the antigenic polypeptide to be presented into a 50ng / mL polypeptide solution;

[0033] 7) Add 1mL of the prepared peptide solution to the incubated PBMC cells;

[0034] 8) Continue to incubate at 37°C for 2 hours;

[0035] 9) Wash the cells once with PBS;

[0036] 10) The incubated PBMC cells and unincubated PBM...

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Abstract

The invention provides a neoantigen-presenting cell for cell therapy and a preparation method thereof, which uses human peripheral blood mononuclear cells to undergo in vitro treatment to make it have a strong antigen-presenting function, and after carrying out antigen loading in vitro, Proliferation of specific T cells was induced. The method is not only simple and fast, but also can obtain a large number of antigen-presenting cells, and the obtained antigen-presenting cells can be loaded with antigens to activate the proliferation of specific T cells. The antigen presentation effect is remarkable, and the rejection reaction is slight when used for treatment, and the safety is high. , no obvious side effects.

Description

technical field [0001] The invention belongs to the field of cell engineering, and particularly relates to a new antigen-presenting cell for cell therapy and a preparation method thereof. Background technique [0002] Antigen-presenting cells (APCs) can ingest and process antigens, present antigens to lymphocytes, and assist and regulate T cells and B cells to recognize and respond to antigens. Professional antigen-presenting cells have a mononuclear phagocytic system, such as monocyte-macrophages and dendritic cells. The content of this type of cells in peripheral blood is low, usually less than 2%, and it is not easy to obtain, and there are limitations such as complex processing and self-proliferation. Non-professional antigen-presenting cells include vascular endothelial cells, epithelial cells, mesenchymal cells, fibroblasts and activated T cells. These cells are usually related to the occurrence of inflammatory reactions and some autoimmune diseases, and are not easy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/078
Inventor 张嵘张天赋
Owner BEIJING DCTY BIOTECH CO LTD
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