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A kind of mesenchymal stem cell proliferation and differentiation culture medium

A technology of mesenchymal stem cells, proliferation and differentiation, applied in the field of stem cells, can solve the problems such as Atractylodes lactone II that has not been disclosed in the research, and achieve the effect of increasing the amount of differentiated bone, promoting osteogenic differentiation, and promoting proliferation

Active Publication Date: 2018-11-23
HUNAN YUANPIN CELL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, no studies have disclosed these activities of atractyloid II

Method used

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  • A kind of mesenchymal stem cell proliferation and differentiation culture medium
  • A kind of mesenchymal stem cell proliferation and differentiation culture medium
  • A kind of mesenchymal stem cell proliferation and differentiation culture medium

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Embodiment Construction

[0019] The substantive content of the present invention will be specifically introduced below in conjunction with the embodiments. Due to space reasons, the description of the experimental process cannot be done in great detail. All the parts not described in detail in the experiment are routine operations well known to those skilled in the art.

[0020] 1. Experimental materials

[0021] Atractylodes Ⅱ, Atractylodes Ⅰ and Atractylodes Ⅲ are self-made, and the purity is greater than 98%.

[0022] DMEM / F12 (1:1) medium was purchased from Hyclone Company as a serum-free medium.

[0023] 2. Experimental method

[0024] 1. Isolation and culture of bone marrow mesenchymal stem cells (BMSCs)

[0025] 14-day-old healthy SD rats were taken out, killed by cervical dislocation and soaked in 75% alcohol for 10 minutes. Under aseptic conditions, bilateral femurs, tibias and humeri were taken out, placed in serum-free culture medium, and bilateral metaphyses were cut off. , to expose th...

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Abstract

The invention discloses a mesenchymal stem cell proliferation and differentiation culture solution with atractylenolide II as a nutrient factor. According to the culture solution, a DMEM / F12 (Dulbecco's Modified Eagle Medium) culture medium solution is adopted as a base culture solution, and the basic culture solution has atractylenolide II of an effective concentration; mesenchymal stem cells areadopted as mesenchymal stem cells; differentiation refers to osteogenesis differentiation; and the concentration of the atractylenolide II is 5-60mu m. Results show that the atractylenolide II is capable of promoting proliferation and osteogenesis differentiation of mesenchymal stem cells and can be adopted to prepare culture solutions for inducing osteogenesis differentiation of the mesenchymalstem cells, and thus the osteogenesis differentiation of the mesenchymal stem cells can be effectively increased.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to the proliferation and differentiation of stem cells, in particular to a culture medium for proliferation and differentiation of mesenchymal stem cells using atractyloid II as a nutrient factor. Background technique [0002] The study of bone tissue engineering provides a new hope for the treatment of large bone defects. Bone tissue engineering consists of three elements: stem cells, nutritional factors and scaffold materials. Good stem cells and trophic factors are crucial to the treatment of bone defects. Therefore, finding stem cells with strong proliferation and differentiation capabilities, having strong trophic factors that induce and promote proliferation and differentiation, and achieving the best bone defect repair effect is the orthopedic department. A hotspot of continuous in-depth research in the field. [0003] Nutritional factors can promote the osteogenesis of mesenchymal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/077
Inventor 王莉胡芳关凯
Owner HUNAN YUANPIN CELL TECH CO LTD
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