Fluorescence collection optical system for flow cytometer

Abstract

The invention discloses a fluorescence collection optical system for a flow cytometer, wherein the fluorescence collection optical system includes a flow chamber, a microobjective, an optical fiber seat, a reflecting mirror, a first optical fiber, a second optical fiber, a first collimating mirror and a second collimating mirror; the reflecting mirror coheres to the left side of the flow chamber,the microobjective is placed at the right side of the flow chamber, and the optical fiber seat is placed at the right side of the microobjective; the first optical fiber is located at one side of theoptical fiber seat, and the second optical fiber is located at the other side of the optical fiber seat; the first optical fiber is connected with the first collimating mirror, and the second opticalfiber is connected with the second collimating mirror; the reflecting mirror is a spherical surface, and the spherical surface is plated with a sliver internal reflection film; the microobjective is alimited long-distance microobjective; by addition of the reflecting mirror, the scattered fluorescence emitted after laser irradiates cells in the flow chamber is collected, and at the same time, a part of the fluorescence is anew converged back to a sphere center after being reflected at the sphere center because the reflecting mirror spherical surface is plated with the reflection film, and isfocused on the optical fiber via the microobjective; the problem of weak fluorescence collection ability of an optical system of a conventional flow cytometer is solved, and the fluorescence collection ability is improved.

Description

technical field [0001] The invention relates to the technical field of biological and medical optical analysis, in particular to a fluorescence collection optical system for flow cytometry. Background technique [0002] Flow cytometry is a detection method for quantitative analysis and sorting of single cells or other biological particles at the functional level, rapidly determining the biological properties of individual cells or organelles, and separating specific cells or organelles from the population Classified collection techniques. Flow cytometer is a device for automatic analysis and sorting of cells, which integrates new high-tech instruments such as cell and molecular biology, fluid mechanics, laser technology, optoelectronic technology, computer technology, and cell fluorescence chemistry technology. Flow cytometers usually use a laser as the light source. The focused and shaped beam is irradiated on the sample flow, and the fluorescently stained cells generate ...

AI Technical Summary

Problems solved by technology

[0003] However, only by increasing the numerical aperture of the collection lens, especially when the numerical aperture is greater than 1.0, will bring some technical problems: First, the lens with a larger numerical aperture will have a correspondingly smaller working distance, but the working distance cannot be smaller than The wall thickness of the flow chamber, otherwise the lens cannot be used; the second is that the larger the collection lens, the design of the entire optical system will become very complicated. Usually, multiple lens combinations are required to achieve achromatic correction. With the increase of lens combinations, The improvement of lens accuracy and assembly accuracy increases the cost and makes assembly difficult

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