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Method for identifying molecules of epimedium sagittatum and epimedium myrianthum

A technology for molecular identification of Epimedium dulcis and Epimedium dulcis is applied in the field of molecular identification of Epimedium dulcis and Tianpingshan Epimedium, which can solve problems such as inaccurate identification, achieve accurate results, shorten identification time, and quickly identify Effect

Inactive Publication Date: 2018-01-23
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The taxonomy of Epimedium sagitta and its close relative species E.myrianthum Stearn has been widely debated, and the identification of them only on the basis of small differences in species morphology can easily lead to inaccurate identification and other problems

Method used

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  • Method for identifying molecules of epimedium sagittatum and epimedium myrianthum
  • Method for identifying molecules of epimedium sagittatum and epimedium myrianthum
  • Method for identifying molecules of epimedium sagittatum and epimedium myrianthum

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Embodiment 1

[0022] 1. Collect 3 original plants of Epimedium sagittata from Anhui Province and Hubei Province; collect 4 original plants of Epimedium Tianpingshan from different regions in Guizhou Province. See Table 1 for details:

[0023] Table 1 Sample information of Epimedium sagittatum and Epimedium chinensis

[0024] serial number

Sample serial number

sample name

collection place

1

JY01

Epimedium sagitta

Anhui Province

2

JY02

Epimedium sagitta

Anhui Province

3

JY03

Epimedium sagitta

Hubei Province

4

TPS01

Epimedium tianpingshan

Guizhou Province

5

TPS02

Epimedium tianpingshan

Guizhou Province

6

TPS03

Epimedium tianpingshan

Guizhou Province

7

TPS04

Epimedium tianpingshan

Guizhou Province

[0025] 2. Extraction of Epimedium sagittatum and Epimedium tianpingshan DNA

[0026] Get the fresh leaves of Epimedium sagittatum and Epimedium chi...

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Abstract

The invention discloses a method for identifying molecules of epimedium sagittatum and epimedium myrianthum. The method comprises the following steps of (1) extracting DNA (deoxyribonucleic acid) of ato-be-tested sample; (2) using the DNA of the to-be-tested sample as a template, and performing PCR (polymerase chain reaction) amplification on a segment containing chloroplast psbA-trnH sequence; (3) splicing the amplified products, so as to obtain the complete chloroplast psbA-trnH sequence; (4) establishing a system development tree with sequenced and spliced psbA-trnH sequence based on a K2Pmodel. The method has the advantages that the suitability is strong, the operation is simple, the epimedium sagittatum and the epimedium myrianthum can be quickly and accurately identified, and a large amount of samples can be identified in short time.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a molecular identification method of Epimedium sagittata and Epimedium tianpingshan. Background technique [0002] Epimedium genus Epimedium L. is a perennial herb. It is an ancient and primitive dicotyledonous plant group. It is in a special taxonomic position in the plant phylogenetic tree. It is also one of the important traditional medicinal plants in my country. It has the functions of nourishing kidney yang, strengthening bones, It has the effect of dispelling rheumatism, and has obvious effects in assisting pregnancy, anti-osteoporosis, improving immunity and inhibiting tumors. The 2015 edition of "Chinese Pharmacopoeia" included 5 kinds of Epimedium: Epimedium E.breviconu Maxim., Epimedium sagittatum (Sieb.et Zucc.) Maxim., Epimedium vellus E.pubescens Maxim. ., E.koreanum Nakai and E.wushanense T.S.Ying. Epimedium plants are very similar in morphol...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
Inventor 庞晓慧郭梦月任莉宋经元陈士林
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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