Compound microbial agent for landfill treatment and preparation method thereof

A technology of compound microbial inoculants and landfill waste, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution, and achieve the effect of reducing emissions and good deodorization effect.

Pending Publication Date: 2018-02-02
SHANGHAI LAITAI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a compound microbial agent for landfill waste treatment and its preparation method, which solves the problem that landfill waste emits a large amount of toxic and harmful gases and causes serious pollution to the environment

Method used

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Examples

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preparation example Construction

[0026] A preparation method for a compound microbial bacterial agent for landfill waste treatment, comprising the following steps:

[0027] (1) According to the mass percentage, 10-30% of thermotolerant Lactobacillus, 5-15% of plantarum Lactobacillus, 20-40% of photosynthetic bacteria, 5-20% of Bacillus subtilis, and 20-40% of Pseudomonas are configured into Composite microbial agent mother liquor;

[0028] (2) According to the mass percentage, 30%-70% of molasses, 0-20% of yeast extract, 0-30% of peptone, 0-3% of sodium chloride, 0-5% of sodium nitrite, and 0.5-1% of trace elements , Calcium chloride 5-13% is configured as a medium, and the pH value of the medium is adjusted in the range of 5.0-6.0 by 20% disodium hydrogen phosphate;

[0029] (3) At a temperature of 25-85°C, place the mother liquor of the composite microbial agent in the culture medium for 18-60 hours to obtain the composite microbial agent.

[0030] In step (3), divide the mother liquor of compound microbi...

Embodiment 1

[0034] A composite microbial bacterial agent for landfill waste treatment, the composite microbial bacterial agent is composed of a composite microbial bacterial agent mother liquor and a culture medium;

[0035] According to the mass percentage, the composite microbial agent mother liquor is composed of 10% heat-resistant Lactobacillus, 15% plantarum Lactobacillus, 40% photosynthetic bacteria, 15% Bacillus subtilis, and 20% Pseudomonas;

[0036] According to the mass percentage, the medium is composed of 30% molasses, 50% yeast extract, 30% peptone, 3% sodium chloride, 5% sodium nitrite, 1% trace elements, and 11% calcium chloride. Disodium hydrogen phosphate adjusts the pH value of the medium in the range of 5.0-6.0;

[0037] Divide the mother liquor of the compound microbial agent into two groups, A and B, and put them into the culture medium respectively. According to the ratio of parts by volume, take 1 part of the mother liquor of group A and incubate at 25°C for 30 hour...

Embodiment 2

[0039] A composite microbial bacterial agent for landfill waste treatment, the composite microbial bacterial agent is composed of a composite microbial bacterial agent mother liquor and a culture medium;

[0040] According to the mass percentage, the composite microbial agent mother liquor is composed of 20% heat-resistant Lactobacillus, 15% plantarum Lactobacillus, 30% photosynthetic bacteria, 15% Bacillus subtilis, and 20% Pseudomonas;

[0041] According to the mass percentage, the medium is composed of molasses 50%, yeast extract 10%, peptone 25%, sodium chloride 2%, sodium nitrite 4%, trace elements 1%, calcium chloride 8%, and passed 20% Disodium hydrogen phosphate adjusts the pH value of the medium in the range of 5.0-6.0;

[0042] Divide the mother liquor of the compound microbial agent into two groups, A and B, and put them into the culture medium respectively. According to the ratio of parts by volume, take 2 parts of the mother liquor of group A and incubate at 36°C ...

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Abstract

The invention discloses a compound microbial agent for landfill treatment and a preparation method thereof, and belongs to the technical field of landfill microorganism treatment. The compound microbial agent comprises a compound microbial agent mother solution and a culture medium. The compound microbial agent mother solution comprises, by mass, 10-30% of heatproof lactobacillus, 5-15% of lactobacillus plantarum, 20-40 parts of photosynthetic bacteria, 5-20% of bacillus subtilis and 20-40% of pseudomonas. The culture medium comprises, by mass, 30-70% of molasses, 0-20% of yeast extract, 0-30%of peptone, 0-3% of sodium chloride, 0-5% of sodium nitrite, 0.5-1% of microelements and 5-13% of calcium chloride. The compound microbial agent has the advantages that the safe and long-lasting landfill treatment reagent is provided; the compound microbial agent can take part in landfill reaction to reduce emission of substantial toxic and harmful gases, has good deodorization effect, is safe, poisonless and nuisanceless and does not have toxic and side effects on environment, thereby being a new reagent for treating landfill pollution.

Description

technical field [0001] The invention relates to a compound microbial agent for landfill waste treatment and a preparation method thereof, belonging to the technical field of landfill waste microbial treatment. Background technique [0002] In recent years, with the rapid growth of population and the acceleration of industrial processes, the massive consumption of natural resources has also brought about environmental problems that cannot be ignored. Human survival and development are facing severe challenges, and environmental protection has been increasingly valued by people. . [0003] At present, the disposal of municipal solid waste and sewage sludge in my country is dominated by sanitary landfill. Landfill is still the mainstream way of waste treatment in my country. Because the landfilled waste contains rich nutrients, it still occurs in the state of landfill. Complex physical and chemical reactions and microbial metabolism continuously emit a large amount of toxic and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B1/00C12R1/225C12R1/25C12R1/125C12R1/38
CPCB09B1/004C12N1/20
Inventor 徐苏愉
Owner SHANGHAI LAITAI BIOTECH
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